The Netherlands Institute of Ecology (NIOO-KNAW) houses 8 long glass aquaria (180 x 40 x 40 cm in length x width x height, 288L) and 24 shorter glass aquaria (90 × 50 × 50 cm in length x width x height, 225L). The aquaria can be kept at different temperatures which can be controlled by an automatic control system (Cascade Automation Systems, Ridderkerk, the Netherlands).

The NIOO greenhouse and trace gas analysis facility is part of the chemical analytical lab with equipment and facilities to asses gas concentrations in atmosphere or in soil gas or soil moisture. Gases (CH4, CO2, N2O, CO, H2,C¬2H4 can be measured over a wide concentration range and even to below-atmospheric levels (ppb) or in stable isotope studies (13C/12C in CO2, CH4).

The Netherlands Institute of Ecology (NIOO-KNAW) houses 36x10L microcosms (transparent polycarbonate carboys; NALGENE, Rochester, USA) for plankton research. The microcosms can be temperature-controlled in water baths (at 0.5 °C temperature resolution), and automatically stirred through magnetic stirrers mounted below the water baths.

Here, we use a latitudinal gradient spanning 3,000 km across Europe to examine bacterial and fungal communities in the rhizosphere and surrounding soils of range-expanding plant species. We selected range-expanding plants with and without congeneric native species in the new range and, as a control, the congeneric native species.

Nematode communities play an important role in soil ecology research. To analyse different type of plant-soil nematodes, NIOO has a facility for extracting soil and root nematodes by Oostenbrink funnel and mistifier.

The NIOO Phytotron is a complex of greenhouses and climate chambers. In addition to offering controlled growing and rearing environments, the Phytotron offers rooms to process, prepare, harvest or store experiments. The Phytotron also provides physiological analysis- and field equipment, ovens and has a large stock of disposables which can be used.

The molecular laboratories offer a wide range of facilities needed for handling, preparing and analysing a wide variety of DNA and/or RNA samples. The lab is well-equipped, and in addition to the general molecular laboratory there are several specialised laboratories such as an MLII Lab, an RNA Lab and a PCR lab with UV-cupboards.

This is a systematic collection of eight plant species from five grassland sites in the Veluwe, for which some tissue from individual plants is stored at -80 °C. For each site, up to 10 individuals from each species have been sampled along a transect at two time points (late May and early August).

NIOO has a collection of thousands of bacterial strains isolated from different (semi-)natural and agricultural environments. Most are bacteria isolated from soils, rhizosphere or plant parts, but aquatic strains are present as well.

The Passerine testing facility offers a unique oportunity to house and measure passerine birds for an array of behavioural tests in a controlled environment.