Melissah Rowe

Dr. Melissah Rowe

Tenure Track

Bezoekadres

Droevendaalsesteeg 10
6708 PB Wageningen

+31 (0) 317 47 34 00

The Netherlands

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Why and how do animals vary in their ability to reproduce?

Biografie

My fascination with sexual selection was sparked during my undergraduate studies (Macquarie University, Australia) when I learned about Jonathan Waage’s work on sperm displacement in damselflies. This set me off on an adventure that has taken me around the world. I completed my PhD at the University of Chicago (USA) and a postdoctoral fellowship at Arizona State University (USA), before moving to Norway to work on sperm evolution at the Natural History Museum of Oslo. In 2014, I became a junior group leader at the University of Oslo, where my group investigated the role of microbes in the functional evolution of avian ejaculates.

In 2019, I joined the Department of Animal Ecology at NIOO-KNAW. Research in my group focuses on understanding why and how animals vary in their ability to reproduce, with a particular focus on understanding the consequences of variation in post-copulatory sexual traits for fitness. We work primarily with passerine birds and use a wide range of methodological and analytical approaches in both field-based studies of natural populations and experimental work in captive populations.

Onderzoeksgroepen

CV

Employment

  • 2019–Present
    Tenure Track Researcher (NIOO-KNAW)
  • 2014–2019
    Researcher at the Centre for Ecological and Evolutionary Synthesis (CEES) and Natural History Museum, University of Oslo (Norway)
  • 2010–2014
    Postdoc at the Natural History Museum, University of Oslo (Norway)
  • 2008–2010
    Postdoc at the School of Life Sciences, Arizona State University (USA)

Education

  • 2005–2008
    PhD in the Department of Ecology and Evolution, University of Chicago (USA)
  • 2003–2005
    MSc in the Department of Ecology and Evolution, University of Chicago (USA)
  • 1998–2000
    BSc (First Class Honours) in the School of Life Sciences, Macquarie University (Australia)

Publicaties

Belangrijkste publicaties

Peer-reviewed publicaties

  • Molecular Ecology
    2022

    Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow

    Homa Papoli Yazdi, Mark Ravinet, Melissah Rowe, Glenn Peter Sætre, Caroline Guldvog, Fabrice Eroukhmanoff, Alfonso Marzal, Sergio Magallanes, Anna Runemark

    Hybridization can result in novel allelic combinations which can impact the hybrid phenotype through changes in gene expression. While misexpression in F1 hybrids is well documented, how gene expression evolves in stabilized hybrid taxa remains an open question. As gene expression evolves in a stabilizing manner, break-up of co-evolved cis- and trans-regulatory elements could lead to transgressive patterns of gene expression in hybrids. Here, we address to what extent gonad gene expression has evolved in an established and stable homoploid hybrid, the Italian sparrow (Passer italiae). Through comparison of gene expression in gonads from individuals of the two parental species (i.e., house and Spanish sparrow) to that of Italian sparrows, we find evidence for strongly transgressive expression in male Italian sparrows—2530 genes (22% of testis genes tested for inheritance) exhibit expression patterns outside the range of both parent species. In contrast, Italian sparrow ovary expression was similar to that of one of the parent species, the house sparrow (Passer domesticus). Moreover, the Italian sparrow testis transcriptome is 26 times as diverged from those of the parent species as the parental transcriptomes are from each other, despite being genetically intermediate. This highlights the potential for regulation of gene expression to produce novel variation following hybridization. Genes involved in mitochondrial respiratory chain complexes and protein synthesis are enriched in the subset that is over-dominantly expressed in Italian sparrow testis, suggesting that selection on key functions has moulded the hybrid Italian sparrow transcriptome.

    https://doi.org/10.1111/mec.16572
  • Cells
    2022

    Sperm Numbers as a Paternity Guard in a Wild Bird

    Melissah Rowe, Annabel van Oort, Lyanne Brouwer, Jan T. Lifjeld, Michael S. Webster, Joseph F. Welklin, Daniel T. Baldassarre

    Sperm competition is thought to impose strong selection on males to produce competitive ejaculates to outcompete rival males under competitive mating conditions. Our understanding of how different sperm traits influence fertilization success, however, remains limited, especially in wild populations. Recent literature highlights the importance of incorporating multiple ejaculate traits and pre-copulatory sexually selected traits in analyses aimed at understanding how selection acts on sperm traits. However, variation in a male’s ability to gain fertilization success may also depend upon a range of social and ecological factors that determine the opportunity for mating events both within and outside of the social pair-bond. Here, we test for an effect of sperm quantity and sperm size on male reproductive success in the red-back fairy-wren (Malurus melanocephalus) while simultaneously accounting for pre-copulatory sexual selection and potential socio-ecological correlates of male mating success. We found that sperm number (i.e., cloacal protuberance volume), but not sperm morphology, was associated with reproductive success in male red-backed fairy-wrens. Most notably, males with large numbers of sperm available for copulation achieved greater within-pair paternity success. Our results suggest that males use large sperm numbers as a defensive strategy to guard within-pair paternity success in a system where there is a high risk of sperm competition and female control of copulation. Finally, our work highlights the importance of accounting for socio-ecological factors that may influence male mating opportunities when examining the role of sperm traits in determining male reproductive success.

    https://doi.org/10.3390/cells11020231
  • Behavioral Ecology and Sociobiology
    27-04-2021

    Sperm Sizer: a program to semi-automate the measurement of sperm length

    Callum S. McDiarmid, Roger Li, Ariel F. Kahrl, Melissah Rowe, Simon C. Griffith
    Research on sperm is incorporated into many areas of ecology and evolution including sexual selection, reproductive physiology and ecotoxicology, as well as comparative studies in evolution and phylogenetics. Currently, producing data on sperm morphology involves several time-consuming steps, particularly photographing sperm and measuring their length (e.g. head, midpiece, tail and total sperm length). Here, we present Sperm Sizer, a freely available Java program that semi-automates the process of measuring sperm length along the centre of the sperm (including head, midpiece, tail and total length). We compare sperm measurements made with Sperm Sizer to those made with the widely used non-automated software ImageJ, for sperm from a single bird species (the long-tailed finch Poephila acuticauda), eight species of passerine bird and eight species of lizard, and provide examples demonstrating that the program can measure at least some mammalian, fish and mollusc sperm. Sperm length measurements from Sperm Sizer are highly correlated to those made using ImageJ, demonstrating that Sperm Sizer produces high quality sperm length data while taking drastically less time. Our data suggests that Sperm Sizer measurements could possibly be incorporated into existing large datasets with a small correction, although this will need to be assessed on a case-by-case basis. We suggest that generally, sperm image quality (high contrast, minimal overlap of sperm, etc.) will be more important than the shape of the sperm for whether or not Sperm Sizer can be employed for a given project.
    https://doi.org/10.1007/s00265-021-03013-4
  • Biological Journal of the Linnean Society
    2021

    Highly variable sperm morphology in the masked finch (Poephila personata) and other estrildid finches

    Elise Mccarthy, Callum S. McDiarmid, Laura L. Hurley, Melissah Rowe, Simon C. Griffith

    Spermatozoa exhibit remarkable levels of morphological diversification among and within species. Among the passerine birds, the zebra finch (Taeniopygia guttata) has become a model system for studies of sperm biology, yet studies of closely related Estrildidae finches remain scarce. Here, we examine sperm morphology in the masked finch (Poephila personata) and place the data into the broader context of passerine sperm morphology using data for an additional 189 species. The masked finch exhibited high levels of within- and among-male variation in total sperm length and in specific sperm components. Furthermore, among-male variance in sperm length was significantly greater in estrildid (N = 12) compared with non-estrildid species (N = 178). We suggest that the high variation in sperm morphology in the masked finch and other estrildid species is likely to be linked to low levels of sperm competition, hence relaxed or weak selection on sperm length, in the clade. Our findings highlight that the highly variable sperm of the masked finch and widely studied zebra finch are 'typical' for estrildid species and stress the relevance of studying groups of closely related species. Finally, we suggest that further studies of Estrildidae will enhance our understanding of sperm diversity and avian diversity more generally.

    https://doi.org/10.1093/biolinnean/blab048
  • PeerJ
    11-11-2020

    Variation in female reproductive tract morphology across the reproductive cycle in the zebra finch

    Laura L. Hurley, O.L. Crino, Melissah Rowe, S.C. Griffith
    Background
    In seasonally breeding birds, the reproductive tract undergoes a dramatic circannual cycle of recrudescence and regression, with oviduct size increasing 5–220 fold from the non-breeding to the breeding state. Opportunistically breeding birds can produce multiple clutches sequentially across an extended period in response primarily to environmental rather than seasonal cues. In the zebra finch, it has been shown that there is a significant reduction in gonadal morphology in non-breeding females. However, the scale of recrudescence and regression of reproductive tissue within a single breeding cycle is unknown and yet important to understand the cost of breeding, and the physiological readiness to breed in such flexible breeders.

    Methods
    We examined the reproductive tissue of breeding female zebra finches at six stages in the nesting cycle from pre-breeding to fledging offspring. We quantified the wet mass of the oviduct, the volume of the largest pre-ovulatory follicle, and the total number of pre-ovulatory follicles present on the ovary.

    Results
    Measures of the female reproductive tract were highest during nesting and laying stages and declined significantly in the later stages of the breeding cycle. Importantly, we found that the mass of reproductive tissue changes as much across a single reproductive event as that previously characterized between birds categorized as breeding and non-breeding. However, the regression of the ovary is less dramatic than that seen in seasonal breeders. This could reflect low-level maintenance of reproductive tissues in opportunistic breeders, but needs to be confirmed in wild non-breeding birds.
    https://doi.org/10.7717/peerj.10195
  • Journal of Avian Biology
    15-09-2020

    Innate and adaptive immune proteins in the preen gland secretions of male house sparrows

    D. Carneiro, G.A. Czirjak, Melissah Rowe
    Recent studies have demonstrated that preen oil acts to reduce or eliminate feather‐associated bacteria. The mechanisms underlying this antibacterial activity, however, are incompletely understood. In addition to the activity of alcohols (i.e. 3,7‐dimethyloctan‐1‐ol), recent research has suggested that antimicrobial peptides may underlie the antibacterial activity of preen oil. Here, we document the presence of innate and adaptive immune proteins, lysozyme and immunoglobulin Y (IgY), in the preen oil of house sparrows Passer domesticus. We suggest lysozyme functions as an antimicrobial agent, with potentially important impacts against Gram‐positive feather degrading bacteria. Furthermore, both lysozyme and IgY likely act in local immune defence of the preen gland, and may also play a role in regulating the local microbiome, with potentially important consequences for chemical communication and signalling. Our findings suggest that the preen gland and its secretions should be considered an integral part of the body's first line of defence against invading infections.
    https://doi.org/10.1111/jav.02556
  • Molecular Biology and Evolution
    2020

    Molecular Diversification of the Seminal Fluid Proteome in a Recently Diverged Passerine Species Pair

    Melissah Rowe, Emma Whittington, Kirill Borziak, Mark Ravinet, Fabrice Eroukhmanoff, S Glenn-Peter, Steve Dorus
    Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held that SFPs may contribute to postmating, prezygotic reproductive barriers between closely related taxa. We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and Passer hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. First, we characterized and compared the SF proteome of the two species, revealing consistencies with known aspects of SFP biology and function in other taxa, including the presence and diversification of proteins involved in immunity and sperm maturation. Second, using whole-genome resequencing data, we assessed patterns of genomic differentiation between house and Spanish sparrows. These analyses detected divergent selection on immunity-related SF genes and positive selective sweeps in regions containing a number of SF genes that also exhibited protein abundance diversification between species. Finally, we analyzed the molecular evolution of SFPs across 11 passerine species and found a significantly higher rate of positive selection in SFPs compared with the rest of the genome, as well as significant enrichments for functional pathways related to immunity in the set of positively selected SF genes. Our results suggest that selection on immunity pathways is an important determinant of passerine SF composition and evolution. Assessing the role of immunity genes in speciation in other recently diverged taxa should be prioritized given the potential role for immunity-related proteins in reproductive incompatibilities in Passer sparrows.
    https://doi.org/10.1093/molbev/msz235
  • Behavioral Ecology and Sociobiology
    2020

    Reproductive coordination breeds success: the importance of the partnership in avian sperm biology

    Laura L. Hurley, Melissah Rowe, Simon C. Griffith
    Previous experience with a partner can improve reproductive coordination between a pair and increase offspring production. We paired inexperienced zebra finches and investigated how a pairs’ experience and their reproductive success together (i.e., whether they were successful or unsuccessful at rearing chicks) related to the number of sperm reaching the ovum, sperm motile performance, and hatching success. In contrast to unsuccessful pairs, successful pairs increased their relative hatching rates over sequential breeding attempts, with pairs hatching 100% of eggs after successfully fledging their previous clutch. Across the study, hatching failure was primarily due to early embryo death. Further, the number of sperm reaching the perivitelline layer (PVL) significantly decreased after fledging chicks in successful pairs, and overall, less sperm was found on the PVL in successful pairs compared with unsuccessful pairs. Across breeding attempts, males in successful pairs also exhibited a significant decline in sperm swimming speed, whereas it increased over breeding attempts in unsuccessful pairs. Our results support the idea of an optimal level of supernumerary sperm on the avian egg. However, our data suggest that there are likely to be interactions between the quality of a partnership and male sperm traits that may contribute to fitness in socially monogamous birds and that have been largely neglected to date.
    https://doi.org/10.1007/s00265-019-2782-9
  • Molecular Reproduction and Development
    2020

    There and back again: A sperm's tale

  • Zoology
    2020

    Sperm length variation among Afrotropical songbirds reflects phylogeny rather than adaptations to the tropical environment

    Taiwo C. Omotoriogun, Tomáš Albrecht, Jostein Gohli, David Hořák, Lars Erik Johannessen, Arild Johnsen, Jakub Kreisinger, Petter Z. Marki, Ulf Ottosson, Melissah Rowe, Ondřej Sedláček, Jan T. Lifjeld
    Sperm cells vary tremendously in size and shape across the animal kingdom. In songbirds (Aves: Passeri), sperm have a characteristic helical form but vary considerably in size. Most of our knowledge about sperm morphology in this group stems from studies of species in the Northern temperate zone, while little is known about the numerous species in the tropics. Here we examined sperm size in 125 Afrotropical songbird species with emphasis on the length of the major structural components (head, midpiece, flagellum), and total sperm length measured using light microscopy. Mean total sperm length varied from 51 μm to 212 μm across species. Those belonging to the Corvoidea superfamily had relatively short sperm with a small midpiece, while those of the three major Passeridan superfamilies Passeroidea, Muscicapoidea and Sylvioidea showed large interspecific variation in total sperm length and associated variation in midpiece length. These patterns are consistent with previous findings for temperate species in the same major clades. A comparative analysis with songbird species from the Northern temperate zone (N = 139) showed large overlap in sperm length ranges although certain temperate families (e.g. Parulidae, Emberizidae) typically have long sperm and certain Afrotropical families (e.g. Cisticolidae, Estrildidae) have relatively short sperm. Afrotropical and temperate species belonging to the same families showed no consistent contrasts in sperm length. Sperm length variation among Afrotropical and Northern temperate songbirds exhibits a strong phylogenetic signal with little or no evidence for any directional latitudinal effect among closely related taxa.
    https://doi.org/10.1016/j.zool.2020.125770

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