Molecular laboratory

The facility

The molecular laboratories offer a wide range of facilities needed for handling, preparing and analysing a wide variety of DNA and/or RNA samples. The lab is well-equipped, and in addition to the general molecular laboratory there are several specialised laboratories such as an MLII Lab, an RNA Lab and a PCR lab with UV-cupboards.

Research topics

The lab is shared and used by all four research departments. There is expertise to work with animal and plant material, viruses and micro-organisms (GMOs).   

References

• Van Gurp, T.P., Wagemaker, N.C.A.M., Wouters, B., Vergeer, P., Ouborg, J.N.J., Verhoeven, K.J.F., 2016. epiGBS: reference-free reduced representation bisulfite sequencing. Nature Methods 13, 322–324.

Running period (guaranteed funding)

Undetermined

Equipment / facilities

• All standard equipment necessary for isolating (high molecular weight) DNA and RNA 
• 2 lysis systems available: Tissue Lyser II (Qiagen) and a FastPrep-24 5G (MP Biomedicals)
• For measuring quality of the DNA or RNA, there is a Nanodrop 2000 (Thermo), Qubit 3.0 (Invitrogen) and a Fragment analyser 5200 (Agilent)
• PCR-machines C1000 Touch (Biorad) and qPCR-machines CFX Touch (Biorad)
• Gel Jet imager (Isogen) to visualise an agrose gel

Data management 

Data derived from analyses conducted at the molecular lab is stored in specific locations on a dedicated bioinformatics server. Data administration is done by data admins for each department. 

Conditions for access

Access is primarily for researchers from NIOO-KNAW. In collaborative projects, the molecular lab can also be used by external researchers. In such cases, an agreement between NIOO and the collaboration partner will be made. Bench fees will apply.