Since 1995, we run a series of biodiversity experiments on a former agricultural field in the nature area Planken-Wambuis near Ede. In some plots, secondary succession has followed its natural course, In other plots, a low or high diversity of plants was sown, or the soil has been inoculated with soil from nature areas.

At Loenderveen, there are 36 outdoor experimental ponds in a 6×6 grid, established in 2005. The ponds are 5m×5m wide at the top and 3m×3m at the bottom, 1.2m deep and lined with waterproof foil. The ponds contain sediment consisting of a 10:1 sand–clay mixture with a depth of 0.3m.

Hoge Veluwe is one of four areas in NIOO-AnE's long-term monitoring research on great tits and other bird species that started in 1955. It consists of a large forest area of more than 350 ha of mainly conifers and oak on poor sandy soil. This area is part of the largest forest complex in the Netherlands, De Veluwe. Every breeding season, many students and researchers are involved in doing field work at Hoge Veluwe.

Liesbos is one of four areas in NIOO-AnE's long-term monitoring research on great tits and other bird species that started in 1955. It consists of a large forest area of about 100 ha of deciduous trees, mainly oak, on rich sandy-loam soil.

Oosterhout is the last of four areas in NIOO-AnE's long-term monitoring research on great tits and other bird species. Research there started in 1956, one year after the other three areas. It consists of deciduous forest of mainly oak trees on a country estate of about 8 ha, and has a rich clay-sandy-loam soil. Oosterhout has around 150 nestboxes.

Vlieland is one of four areas in NIOO-AnE's long-term monitoring research on great tits and other bird species that started in 1955. It consists of several smaller forest areas, which together cover about 250 ha of mainly conifers and oak on poor sandy soil. As Vlieland is an island in the Wadden Sea, the area stands completely on its own. Important population questions in NIOO's long term research are/were studied here.

The foundation 'Land van Ons' has obtained a peat meadow area near Leiden to increase biodiversity in this intensively used agricultural area. The goal is not to recreate nature, but to develop sustainable agricultural use of this land with the goal of increasing biodiversity in the area and developing management strategies that optimise its ecological, economic and recreational value.

The Marker Wadden is a newly constructed archipelago in lake Markermeer, which aims to improve the lake’s degrading food web by stimulating primary productivity. The archipelago consists of five islands that add a currently missing habitat type to the lake: shallow, sheltered waters with high nutrient availability and gradual land-water transitions.

A total of 40 mesocosms are distributed in five rows of eight mesocosms with a spacing of 0.5 m between them. In spring/summer we grow plant species that are currently expanding their range within Europe and species that belong to the same genus while being native in the Netherlands.

Two of the most pervasive human impacts on ecosystems are alteration of global nutrient budgets and changes in the abundance and identity of consumers. In spite of the global impacts of these human activities, there have been no globally coordinated experiments to quantify the general impacts on ecological systems. The Nutrient Network (NutNet) is a grassroots, global research effort to address these questions within a coordinated research network comprised of more than 130 grassland sites worldwide.

A fenced terrain (2500 m2; 80 x 32m) for performing outdoor mesoscosm experiments throughout the year, in the immediate promixity of the NIOO Aquatic Laboratories. Experiments can be performed with > 100 experimental units. There is great flexibility in the spatial set up of experimental units.

The ecotron consists of 60 cilinders with intact (non-disturbed) sand, peat or sea clay cores (20 each). For each soil treatment, ten of the cilinders are inoculated with soil from species-rich grassland with the same soil type. The other ten are inoculated with sterile soil from the same grassland. The cilinders are buried in an experimental garden.