Brazil's Atlantic rainforest biome has lost around 87% of its natural vegetation. Most of those areas were converted to pastures and sugarcane plantations. In this sense, the remaining natural forests, or those restored, are fundamental for the region's ecosystem's functioning. So, the project aims to evaluate the effects of forest restoration methodologies on soil microbial and micro-faunal communities in Atlantic Forest regions, including nematodes. We want to access their structure, composition, potential functions, and interactions in this complex environment. Furthermore, we want to integrate above and below-ground knowledge to optimize forest restoration projects. This data can be crucial knowledge to leverage political, financial, and societal support to encourage and expand the restoration of the Brazilian Atlantic Forest.