Jose G. Maciá-Vicente

Dr. Jose G. Maciá-Vicente

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Visiting Address

Droevendaalsesteeg 10
6708 PB Wageningen

+31 (0) 317 47 34 00

The Netherlands

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About

As a member of the Promise II team at NIOO, my goal is to develop fungal-derived products for effectively controlling Striga, a genus of plant-parasitic weeds that have devastating effects on cereal production in sub-Saharan Africa.

Biography

My main expertise lies in fungal biology, microbial ecology, and phytopathology. My research has primarily focused on investigating the diversity and ecology of fungi associated with plant roots, with the aim of addressing questions regarding their impact on plant growth and ecosystem functions, and the evolutionary pathways leading to their symbiotic relationships. Additionally, I have conducted both fundamental and applied research in areas such as microbiomes, fungal systematics, biological control, and natural products discovery.

Research groups

Publications

Peer-reviewed publications

  • Persoonia
    30-12-2023

    Fungal Planet description sheets: 1550-1613

    Pedro W. Crous, Marco Costa, Hazal Kandemir, Marjan Vermaas, Duong Vu, L. Zhao, E. Arumugam, Adam Flakus, Ž. Jurjević, M. Kaliyaperumal, S. Mahadevakumar, R. Murugadoss, Roger G. Shivas, Y.P. Tan, Michael J. Wingfield, S.E. Abell, T.S. Marney, C. Danteswari, V. Darmostuk, C.M. Denchev, T.T. Denchev, Javier Etayo, J. Gené, S. Gunaseelan, V. Hubka, T. Illescas, G.M. Jansen, K. Kezo, S. Kumar, E. Larsson, K.T. Mufeeda, Marcin Piątek, Pamela Rodriguez-Flakus, P.V.S.R.N. Sarma, M. Stryjak-Bogacka, D. Torres-Garcia, Jukka Vauras, D.A. Acal, A. Akulov, K. Alhudaib, M. Asif, S. Balashov, Hans-Otto Baral, A. Baturo-Cieśniewska, D. Begerow, A. Beja-Pereira, M. Virginia Bianchinotti, P. Bilański, S. Chandranayaka, N. Chellappan, D.A. Cowan, F.A. Custódio, P. Czachura, G. Delgado, Nimali Indeewari de Silva, Jan Dijksterhuis, Margarita Dueñas, P. Eisvand, V. Fachada, J. Fournier, Y. Fritsche, F. Fuljer, K.G.G.. Ganga, M.P. Guerra, K. Hansen, N.L. Hywel-Jones, A M Ismail, C.R. Jacobs, R. Jankowiak, A. Karich, Martin Kemler, K. Kisło, W. Klofac, Irmgard Krisai-Greilhuber, K.P.D. Latha, R Lebeuf, M.E. Lopes, S. Lumyong, Jose Maciá Vicente, G. Maggs-Kölling, D. Magistà, P. Manimohan, María P Martín, E. Mazur, M. Mehrabi-Koushki, Andrew N. Miller, A. Mombert, E.A. Ossowska, K. Patejuk, Olinto L. Pereira, S. Piskorski, Mireia Plaza, A.R. Podile, A. Polhorský, W. Pusz, M. Raza, M. Ruszkiewicz-Michalska, Malka Saba, R.M. Sánchez, R. Singh, L. Śliwa, Maudy Smith, V.M. Stefenon, D. Strašiftáková, N. Suwannarach, K. Szczepańska, M Teresa Telleria, Danushka S. Tennakoon, M. Thines, R Greg Thorn, J. Urbaniak, M. van der Vegte, V. Vasan, C. Vila-Viçosa, Hermann Voglmayr, M. Wrzosek, J. Zappelini, Ewald Groenewald
    Novel species of fungi described in this study include those from various countries as follows: Argentina, Neocamarosporium halophilum in leaf spots of Atriplex undulata. Australia, Aschersonia merianiae on scale insect (Coccoidea), Curvularia huamulaniae isolated from air, Hevansia mainiae on dead spider, Ophiocordyceps poecilometigena on Poecilometis sp. Bolivia, Lecanora menthoides on sandstone, in open semi-desert montane areas, Sticta monlueckiorum corticolous in a forest, Trichonectria epimegalosporae on apothecia of corticolous Megalospora sulphurata var. sulphurata, Trichonectria puncteliae on the thallus of Punctelia borreri. Brazil, Catenomargarita pseudocercosporicola (incl. Catenomargarita gen. nov.) hyperparasitic on Pseudocercospora fijiensis on leaves of Musa acuminata, Tulasnella restingae on protocorms and roots of Epidendrum fulgens. Bulgaria, Anthracoidea umbrosae on Carex spp. Croatia, Hymenoscyphus radicis from surface-sterilised, asymptomatic roots of Microthlaspi erraticum, Orbilia multiserpentina on wood of decorticated branches of Quercus pubescens. France, Calosporella punctatispora on dead corticated twigs of Acer opalus. French West Indies (Martinique), Eutypella lechatii on dead corticated palm stem. Germany, Arrhenia alcalinophila on loamy soil. Iceland, Cistella blauvikensis on dead grass (Poaceae). India, Fulvifomes maritimus on living Peltophorum pterocarpum, Fulvifomes natarajanii on dead wood of Prosopis juliflora, Fulvifomes subazonatus on trunk of Azadirachta indica, Macrolepiota bharadwajii on moist soil near the forest, Narcissea delicata on decaying elephant dung, Paramyrothecium indicum on living leaves of Hibiscus hispidissimus, Trichoglossum syamviswanathii on moist soil near the base of a bamboo plantation. Iran, Vacuiphoma astragalicola from stem canker of Astragalus sarcocolla. Malaysia, Neoeriomycopsis fissistigmae (incl. Neoeriomycopsidaceae fam. nov.) on leaf spots on flower Fissistigma sp. Namibia, Exophiala lichenicola lichenico­lous on Acarospora cf. luederitzensis. Netherlands, Entoloma occultatum on soil, Extremus caricis on dead leaves of Carex sp., Inocybe pseudomytiliodora on loamy soil. Norway, Inocybe guldeniae on calcareous soil, Inocybe rupestroides on gravelly soil. Pakistan, Hymenagaricus brunneodiscus on soil. Philippines, Ophiocordyceps philippinensis parasitic on Asilus sp. Poland, Hawksworthiomyces ciconiae isolated from Ciconia ciconia nest, Plectosphaerella vigrensis from leaf spots on Impatiens noli-tangere, Xenoramularia epitaxicola from sooty mould community on Taxus baccata. Portugal, Inocybe dagamae on clay soil. Saudi Arabia, Diaporthe jazanensis on branches of Coffea arabica. South Africa, Alternaria moraeae on dead leaves of Moraea sp., Bonitomyces buffels­kloofinus (incl. Bonitomyces gen. nov.) on dead twigs of unknown tree, Constrictochalara koukolii on living leaves of Itea rhamnoides colonised by a Meliola sp., Cylindromonium lichenophilum on Parmelina tiliacea, Gamszarella buffelskloofina (incl. Gamszarella gen. nov.) on dead insect, Isthmosporiella africana (incl. Isthmosporiella gen. nov.) on dead twigs of unknown tree, Nothoeucasphaeria buffelskloofina (incl. Nothoeucasphaeria gen. nov.), on dead twigs of unknown tree, Nothomicrothyrium beaucarneae (incl. Nothomicrothyrium gen. nov.) on dead leaves of Beaucarnea stricta, Paramycosphaerella proteae on living leaves of Protea caffra, Querciphoma foliicola on leaf litter, Rachicladosporium conostomii on dead twigs of Conostomium natalense var. glabrum, Rhamphoriopsis synnematosa on dead twig of unknown tree, Waltergamsia mpumalanga on dead leaves of unknown tree. Spain, Amanita fulvogrisea on limestone soil, in mixed forest, Amanita herculis in open Quercus forest, Vuilleminia beltraniae on Cistus symphytifolius. Sweden, Pachyella pulchella on decaying wood on sand-silt riverbank. Thailand, Deniquelata cassiae on dead stem of Cassia fistula, Stomiopeltis thailandica on dead twigs of Magnolia champaca. Ukraine, Circinaria podoliana on natural limestone outcrops, Neonematogonum carpinicola (incl. Neonematogonum gen. nov.) on dead branches of Carpinus betulus. USA, Exophiala wilsonii water from cooling tower, Hygrophorus aesculeticola on soil in mixed forest, and Neocelosporium aereum from air in a house attic. Morphological and culture characteristics are supported by DNA barcodes.
    https://doi.org/doi:10.3767/persoonia.2023.51.08
  • Science advances
    12-2023

    Connecting the multiple dimensions of global soil fungal diversity

    Vladimir S. Mikryukov, Olesya Dulya, Alexander Zizka, Mohammad Bahram, Niloufar Hagh-Doust, Sten Anslan, Oleh Prylutskyi, Manuel Delgado-Baquerizo, Fernando T. Maestre, R. Henrik Nilsson, Jaan Pärn, Maarja Öpik, Mari Moora, Martin Zobel, Mikk Espenberg, Ülo Mander, Abdul Nasir Khalid, Adriana Corrales, Ahto Agan, Aida Marcela Vasco-Palacios, Alessandro Saitta, Andrea Rinaldi, Annemieke Verbeken, Bobby P. Sulistyo, Boris Tamgnoue, Brendan Furneaux, Camila Duarte Ritter, Casper Nyamukondiwa, Cathy Sharp, César Marín, Daniyal Gohar, Darta Klavina, Dipon Sharmah, Dong-Qin Dai, Eduardo Nouhra, Elisabeth Machteld Biersma, Elisabeth Rähn, Erin K. Cameron, Eske De Crop, Eveli Otsing, Evgeny A. Davydov, Felipe E. Albornoz, Francis Q. Brearley, Franz Buegger, Geoffrey Zahn, Gregory Bonito, Inga Hiiesalu, Isabel C. Barrio, Jacob Heilmann-Clausen, Jelena Ankuda, Jiri Doležal, John Y. Kupagme, Jose Maciá Vicente, Joseph Djeugap Fovo, József Geml, Juha M. Alatalo, Julieta Alvarez-Manjarrez, Kadri Põldmaa, Kadri Runnel, Kalev Adamson, Kari Anne Bråthen, Karin Pritsch, Kassim Tchan Issifou, Kęstutis Armolaitis, Kevin David Hyde, Kevin K. Newsham, Kristel Panksep, Adebola Azeez Lateef, Linda Hansson, Louis J. Lamit, Malka Saba, Maria Tuomi, Marieka Gryzenhout, Marijn Bauters, Meike Piepenbring, Nalin N. Wijayawardene, Nourou S. Yorou, Olavi Kurina, Peter E. Mortimer, Peter Meidl, Petr Kohout, Rasmus Puusepp, Rein Drenkhan, Roberto Garibay-Orijel, Roberto Godoy, Saad Alkahtani, Saleh Rahimlou, Sergey V. Dudov, Sergei Põlme, Soumya Ghosh, Sunil Mundra, Talaat Ahmed, Tarquin Netherway, Terry W. Henkel, Tomas L. Roslin, Vincent Nteziryayo, Vladimir E. Fedosov, Vladimir G. Onipchenko, Weeragalle Arachchillage Erandi Yasanthika, Young Woon Lim, Michael E. van Nuland, Nadejda A. Soudzilovskaia, Alexandre Antonelli, Urmas Kõljalg, Kessy Abarenkov, Leho Tedersoo

    How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different levels of taxonomic and phylogenetic diversity of fungi and their ecological groups. We show the impact of precipitation and temperature interactions on local fungal species richness (alpha diversity) across different climates. Our findings reveal how temperature drives fungal compositional turnover (beta diversity) and phylogenetic diversity, linking them with regional species richness (gamma diversity). We integrate fungi into the principles of global biodiversity distribution and present detailed maps for biodiversity conservation and modeling of global ecological processes.

    https://doi.org/10.1126/sciadv.adj8016
  • CABI Agriculture and Bioscience
    12-2023

    Mind the blind spot

    Mengshuai Liu, Jose Maciá Vicente, Jasper van Ruijven, Wopke van der Werf, Zhenling Cui, Fusuo Zhang, Chunxu Song, Liesje Mommer

    Background: Plant–soil feedback (PSF) has gained increasing interest in agricultural systems. An important question is whether PSF differs between different cropping systems. Few attempts have yet been made to identify the pathogen species involved in negative PSF. Here, we hypothesize that the strength of negative PSF experienced by a crop species is determined by the relative abundance of host-specific soil-borne pathogenic fungi, that is in turn driven by the crop’s relative abundance (in time). Methods: We performed a PSF experiment, with different soils originating from three cropping systems in the North China Plain and three crop species (wheat, maize, soybean) in a full factorial design. Soil fungal community composition and relative abundance of fungal (pathogen) species in each treatment was identified by metabarcoding using ITS (Internal Transcribed Spacer) sequencing. Results: PSF ranged from negative for wheat, neutral to negative for soybean and neutral to positive for maize, but the former density of a crop in a particular cropping system did not affect the strength of PSF experienced by each of the three. No relationships between fungal pathogen abundance and PSF were found, but we did find a surprisingly large enrichment across steps of the experiment of Chaetomium spp., a known cellulose-degrading fungus. This may be explained by addition of filter paper on the bottom of the pots. Conclusions: Our results suggest that the strength of PSF in these crops is not related to the relative abundance of specific fungal pathogens. However, we cannot rule out that our results were affected by the high abundance of one particular cellulose-degrading fungus. This highlights both the need to stop the practice of using filter paper in pot experiments, as well as the relevance of assessing the identity, relative abundance and potential functions of fungal taxa in PSF experiments.

    https://doi.org/10.1186/s43170-023-00147-5
  • Persoonia: Molecular Phylogeny and Evolution of Fungi
    12-2023

    Fungal planet description sheets

    Pedro W. Crous, Marco Costa, Hazal Kandemir, Marjan Vermaas, Duong Vu, L. Zhao, E. Arumugam, Adam Flakus, Ž. Jurjević, M. Kaliyaperumal, S. Mahadevakumar, R. Murugadoss, Roger G. Shivas, Y.P. Tan, Michael J. Wingfield, S.E. Abell, T.S. Marney, C. Danteswari, V. Darmostuk, C.M. Denchev, T.T. Denchev, Javier Etayo, J. Gené, S. Gunaseelan, V. Hubka, T. Illescas, G.M. Jansen, K. Kezo, S. Kumar, E. Larsson, K.T. Mufeeda, Marcin Piątek, Pamela Rodriguez-Flakus, P.V.S.R.N. Sarma, M. Stryjak-Bogacka, D. Torres-Garcia, Jukka Vauras, D.A. Acal, A. Akulov, K. Alhudaib, M. Asif, S. Balashov, Hans-Otto Baral, A. Baturo-Cieśniewska, D. Begerow, A. Beja-Pereira, M. Virginia Bianchinotti, P. Bilański, S. Chandranayaka, N. Chellappan, D.A. Cowan, F.A. Custódio, P. Czachura, G. Delgado, Nimali Indeewari de Silva, Jan Dijksterhuis, Margarita Duenas, P. Eisvand, V. Fachada, J. Fournier, Y. Fritsche, F. Fuljer, K.G.G.. Ganga, M.P. Guerra, K. Hansen, N.L. Hywel-Jones, A M Ismail, C.R. Jacobs, R. Jankowiak, A. Karich, M. Kemler, K. Kisło, W. Klofac, Irmgard Krisai-Greilhuber, K.P.D. Latha, R Lebeuf, M.E. Lopes, S. Lumyong, Jose Maciá Vicente, G. Maggs-Kölling, Donato Magistà, P. Manimohan, María P Martín, E. Mazur, M. Mehrabi-Koushki, Andrew N. Miller, A. Mombert, E.A. Ossowska, K. Patejuk, Olinto L. Pereira, S. Piskorski, Mireia Plaza, A.R. Podile, A. Polhorsky, W. Pusz, M. Raza, M. Ruszkiewicz-Michalska, Malka Saba, R.M. Sánchez, R. Singh, L. Śliwa, Maudy Smith, V.M. Stefenon, D. Strasiftáková, N. Suwannarach, K. Szczepańska, M Teresa Telleria, Danushka S. Tennakoon, M. Thines, R Greg Thorn, J. Urbaniak, M. van der Vegte, V. Vasan, C. Vila-Viçosa, Hermann Voglmayr, M. Wrzosek, J. Zappelini, Ewald Groenewald

    Novel species of fungi described in this study include those from various countries as follows: Argen­tina, Neocamarosporium halophilum in leaf spots of Atriplex undulata. Australia, Aschersonia merianiae on scale insect (Coccoidea), Curvularia huamulaniae isolated from air, Hevansia mainiae on dead spider, Ophiocordyceps poecilometigena on Poecilometis sp. Bolivia, Lecanora menthoides on sandstone, in open semi-desert montane areas, Sticta monlueckiorum corticolous in a forest, Trichonectria epimegalosporae on apothecia of corticolous Mega- lospora sulphurata var. sulphurata, Trichonectria puncteliae on the thallus of Punctelia borreri. Brazil, Catenomargarita pseudocercosporicola (incl. Catenomargarita gen. nov.) hyperparasitic on Pseudocercospora fijiensis on leaves of Musa acuminata, Tulasnella restingae on protocorms and roots of Epidendrum fulgens. Bulgaria, Anthracoidea umbrosae on Carex spp. Croatia, Hymenoscyphus radicis from surface-sterilised, asymptomatic roots of Microthlaspi erraticum, Orbilia multiserpentina on wood of decorticated branches of Quercus pubescens. France, Calosporella punctatispora on dead corticated twigs of Acer opalus. French West Indies (Martinique), Eutypella lechatii on dead corticated palm stem. Germany, Arrhenia alcalinophila on loamy soil. Iceland, Cistella blauvikensis on dead grass (Poaceae). India, Fulvifomes maritimus on living Peltophorum pterocarpum, Fulvifomes natarajanii on dead wood of Prosopis juliflora, Fulvifomes subazonatus on trunk of Azadirachta indica, Macrolepiota bharadwajii on moist soil near the forest, Narcissea delicata on decaying elephant dung, Paramyrothecium indicum on living leaves of Hibiscus hispidissimus, Trichoglossum syamviswanathii on moist soil near the base of a bamboo plantation. Iran, Vacuiphoma astragalicola from stem canker of Astragalus sarcocolla. Malaysia, Neoeriomycopsis fissistigmae (incl. Neoeriomycopsidaceae fam. nov.) on leaf spots on flower Fissistigma sp. Namibia, Exophiala lichenicola lichenico- lous on Acarospora cf. luederitzensis. Netherlands, Entoloma occultatum on soil, Extremus caricis on dead leaves of Carex sp., Inocybe pseudomytiliodora on loamy soil. Norway, Inocybe guldeniae on calcareous soil, Inocybe rupestroides on gravelly soil. Pakistan, Hymenagaricus brunneodiscus on soil. Philippines, Ophiocordyceps philippinensis parasitic on Asilus sp. Poland, Hawksworthiomyces ciconiae isolated from Ciconia ciconia nest, Plectosphaerella vigrensis from leaf spots on Impatiens noli-tangere, Xenoramularia epitaxicola from sooty mould community on Taxus baccata. Portugal, Inocybe dagamae on clay soil. Saudi Arabia, Diaporthe jazanensis on branches of Coffea arabica. South Africa, Alternaria moraeae on dead leaves of Moraea sp., Bonitomyces buffels- kloofinus (incl. Bonitomyces gen. nov.) on dead twigs of unknown tree, Constrictochalara koukolii on living leaves of Itea rhamnoides colonised by a Meliola sp., Cylindromonium lichenophilum on Parmelina tiliacea, Gamszarella buffelskloofina (incl. Gamszarella gen. nov.) on dead insect, Isthmosporiella africana (incl. Isthmosporiella gen. nov.) on dead twigs of unknown tree, Nothoeucasphaeria buffelskloofina (incl. Nothoeucasphaeria gen. nov.), on dead twigs of unknown tree, Nothomicrothyrium beaucarneae (incl. Nothomicrothyrium gen. nov.) on dead leaves of Beaucarnea stricta, Paramycosphaerella proteae on living leaves of Protea caffra, Querciphoma foliicola on leaf litter, Rachicladosporium conostomii on dead twigs of Conostomium natalense var. glabrum, Rhamphoriopsis synnematosa on dead twig of unknown tree, Waltergamsia mpumalanga on dead leaves of unknown tree. Spain, Amanita fulvogrisea on limestone soil, in mixed forest, Amanita herculis in open Quercus forest, Vuilleminia beltra- niae on Cistus symphytifolius. Sweden, Pachyella pulchella on decaying wood on sand-silt riverbank. Thailand, Deniquelata cassiae on dead stem of Cassia fistula, Stomiopeltis thailandica on dead twigs of Magnolia champaca. Ukraine, Circinaria podoliana on natural limestone outcrops, Neonematogonum carpinicola (incl. Neonematogonum gen. nov.) on dead branches of Carpinus betulus. USA, Exophiala wilsonii water from cooling tower, Hygrophorus aesculeticola on soil in mixed forest, and Neocelosporium aereum from air in a house attic. Morphological and culture characteristics are supported by DNA barcodes.

    https://doi.org/10.3767/persoonia.2023.51.08
  • European Journal of Plant Pathology
    10-11-2023

    Unraveling the prevalence of soil-borne fungal pathogens in the North China Plain

    Mengshuai Liu, Liesje Mommer, Jasper van Ruijven, Wopke van der Werf, Zhan Xu, Fusuo Zhang, Chunxu Song, Jose Maciá Vicente

    Soil-borne fungal plant pathogens lead to worldwide economic yield losses. However, despite the agricultural importance of the North China Plain (NCP), little is known about the occurrence and severity of soil-borne fungal pathogens that could potentially affect the yields of three main crops in this area: wheat, maize and soybean. By combining searches in public databases, we identified a set of main soil-borne fungal pathogen species potentially affecting crop production in the NCP. We investigated their distribution patterns at three scales: globally, in China and in the NCP, and evaluated how these patterns were determined by a range of variables related to climate, spatial drivers and crop area density of the three crop species. We found 25 main soil-borne fungal pathogens that could potentially affect the yields of wheat, maize and soybean in the NCP. Twenty of these fungal pathogens were prevalent across the globe, and 13 were very frequent in the NCP. Significant positive relationships between pathogen prevalence and crop area density were only found for two specialist pathogens. Our work provides an overview of the main soil-borne fungal pathogens in one of China’s most intensively cultivated regions, shedding light on potential, previously overlooked, fungal threats for agricultural production in the area. This baseline information may contribute to the development of sustainable management strategies aimed at mitigating disease outbreaks caused by soil-borne pathogens in the future.

    https://doi.org/10.1007/s10658-023-02790-0
  • The Cuban Scientist
    22-10-2023

    Uncovering the biodiversity of root endophytic fungi in Europe

    Gregorio Delgado, Jose Maciá Vicente
    Extensive sampling of root endophytic fungi across Europe led to the discovery of two novel fungal genera, Endoradiciella and Extremopsis, and the description of six species new to science.
  • Applied Soil Ecology
    10-2023

    Pinpointing the distinctive impacts of ten cover crop species on the resident and active fractions of the soil microbiome

    Sara G. Cazzaniga, Lize Braat, Sven J.J. van den Elsen, Carin Lombaers, Johnny H.M. Visser, Lia Obinu, Jose Maciá Vicente, Joeke Postma, Liesje Mommer, Hans Helder
    Cover crops are used in agriculture to minimize soil erosion, prevent nutrient leaching and increase soil organic matter content. Cover crops can also be grown to stimulate the soil microbial community to improve soil biological conditions. Despite their widespread use, little is known about the impact of different cover crop species on the composition and activity of the soil microbiome. Here we investigate the effect of distinct cover crop species on the rhizosphere microbiome and characterize both the resident (ribosomal (r)DNA-based) and the potentially active (rRNA-based) fractions of the bacterial, fungal, protist and metazoan communities in the cover crops rhizosphere. We conducted a field experiment using 70-l bottomless containers in which we grew ten monocultures of commonly used cover crop species belonging to five plant families, and an unplanted control treatment (fallow). The total DNA and RNA were extracted from soil and the bacterial, fungal, protistan and metazoan communities were characterized using Illumina MiSeq sequencing. We found that all cover crop species significantly impacted the resident and the potentially active microbial communities in their rhizospheres. Cover crops exerted distinct selection strengths on the native microbial communities. For individual cover crops, the impacts on the resident and the potentially active microbial communities differed while showing similar overall tendencies. Oilseed radish (Brassicaceae) was shown to provoke the strongest microbial shifts, in part attributable to a promotion of the bacterial family Pseudomonadaceae and a repression of Microascaceae in the rhizosphere. Lentil (Fabaceae) induced a widespread stimulation of fungal taxa, including Trichocomaceae and fungal members of the Glomerales order, whereas black oat and hybrid ryegrass (both Poaceae) gave rise to relatively mild changes in the soil microbial communities. Analyses of rRNA-based rhizobiome data revealed that, except for phacelia, all cover crops induced an increase in microbial network complexity as compared to the fallow control. Data presented here provide a broad baseline for the effects of cover crops on four organismal groups, which may facilitate future cover crop selection to advance soil health.
    https://doi.org/10.1016/j.apsoil.2023.105012
  • Molecular Ecology
    07-2023

    The structure of root‐associated fungal communities is related to the long‐term effects of plant diversity on productivity

    Jose Maciá Vicente, Davide Francioli, Alexandra Weigelt, Cynthia Albracht, Kathryn E. Barry, François Buscot, Anne Ebeling, Nico Eisenhauer, Justus Hennecke, Anna Heintz‐Buschart, Jasper van Ruijven, Liesje Mommer
    https://doi.org/10.1111/mec.16956
  • Persoonia
    06-2023

    Fungal Planet description sheets: 1478-1549

    Pedro W. Crous, E.R. Osieck, Roger G. Shivas, Y.P. Tan, S.L. Bishop-Hurley, F. Esteve-Raventos, E. Larsson, Janet Jennifer Luangsa-ard, F. Pancorbo, S. Balashov, I.G. Baseia, T. Boekhout, S. Chandranayaka, D.A. Cowan, R.H.S.F. Cruz, P. Czachura, S. De la Peña-Lastra, F. Dovana, B. Drury, J.W. Fell, Adam Flakus, R. Fotedar, . Jurjevi, A. Kolecka, J. Leebens-Mack, G. Maggs-Kölling, S. Mahadevakumar, A. Mateos, S. Mongkolsamrit, W. Noisripoom, Mireia Plaza, D.P. Overy, M. Pitek, Marcelo Sandoval, Jukka Vauras, Michael J. Wingfield, S.E. Abell, A. Ahmadpour, A. Akulov, F. Alavi, Z. Alavi, A Altes, P. Alvarado, G. Anand, N. Ashtekar, Boris Assyov, G. Banc-Prandi, K.D. Barbosa, G.G. Barreto, J.-M. Bellanger, Jose L. Bezerra, D. Jayarama Bhat, P. Bilaski, Tanay Bose, F. Bozok, J. Chaves, D.H.. Costa-Rezende, C. Danteswari, V. Darmostuk, G. Delgado, S. Denman, A. Eichmeier, Javier Etayo, Guillaume Eyssartier, S. Faulwetter, K.G.G.. Ganga, Y. Ghosta, Marcus J.C. Goh, J.S. Gois, D. Gramaje, L. Granit, Marizeth Groenewald, Gro Gulden, L.F.P. Gusmão, A Hammerbacher, Z. Heidarian, N.L. Hywel-Jones, R. Jankowiak, M. Kaliyaperumal, O. Kaygusuz, K. Kezo, A Khonsanit, S. Kumar, Chang-Hsin Kuo, Laess T., K.P.D. Latha, M. Loizides, S.M. Luo, Jose Maciá Vicente, P. Manimohan, P. A. S. Marbach, P. Marinho, T.S. Marney, Guilhermina Marques, M.P. Martın, Andrew N. Miller, F. Mondello, G. Moreno, K.T. Mufeeda, Hye Yeon Mun, T. Nau, T. Nkomo, A. Okrasiska, J.P.A.F. Oliveira, R.L. Oliveira, D.A. Ortiz, J. Pawowska, M.`A. Perez-De-Gregorio, A.R. Podile, A. Portugal, N. Privitera, Kunhiraman C. Rajeshkumar, I. Rauf, B. Rian, A. Rigueiro-Rodrıguez, G.F.. Rivas-Torres, Pamela Rodriguez-Flakus, M. Romero-Gordillo, Irja Saar, Malka Saba, Daniel W C L Santos, P.V.S.R.N. Sarma, J.L. Siquier, S. Sleiman, M. Spetik, K.R. Sridhar, M. Stryjak-Bogacka, K. Szczepaska, H. Taikn, Danushka S. Tennakoon, D. Thanakitpipattana, J. Trovão, A. Türkekul, A.L. van Iperen, Pieter van 't Hof, G. Vasquez, C.M. Visagie, Brenda D Wingfield, P. T. W. Wong, W.X. Yang, M. Yarar, O. Yarden, N. Yilmaz, N. Zhang, Y.N. Zhu, Ewald Groenewald
    Novel species of fungi described in this study include those from various countries as follows: Australia, Aschersonia mackerrasiae on whitefly, Cladosporium corticola on bark of Melaleuca quinquenervia, Penicillium nudgee from soil under Melaleuca quinquenervia, Pseudocercospora blackwoodiae on leaf spot of Persoonia falcata, and Pseudocercospora dalyelliae on leaf spot of Senna alata. Bolivia, Aspicilia lutzoniana on fully submersed siliceous schist in high-mountain streams, and Niesslia parviseta on the lower part and apothecial discs of Erioderma barbellatum onatwig. Brazil, Cyathus bonsai on decaying wood, Geastrum albofibrosum from moist soil with leaf litter, Laetiporus pratigiensis on a trunk of a living unknown hardwood tree species, and Scytalidium synnematicum on dead twigs of unidentified plant. Bulgaria, Amanita abscondita on sandy soil in a plantation of Quercus suber. Canada, Penicillium acericola on dead bark of Acer saccharum, and Penicillium corticola on dead bark of Acer saccharum. China, Colletotrichum qingyuanense on fruit lesion of Capsicum annuum. Denmark, Helminthosphaeria leptospora on corticioid Neohypochnicium cremicolor. Ecuador (Galapagos), Phaeosphaeria scalesiae on Scalesia sp. Finland, Inocybe jacobssonii on calcareouss oils in dry forests and park habitats. France, Cortinarius rufomyrrheus on sandy soil under Pinus pinaster, and Periconia neominutissima on leaves of Poaceae. India, Coprinopsis fragilis on decaying bark of logs, Filoboletus keralensis on unidentified woody substrate, Penicillium sankaranii from soil, Physisporinus tamilnaduensis on the trunk of Azadirachta indica, and Poronia nagaraholensis on elephant dung. Iran, Neosetophoma fic on infected leaves of Ficus elastica. Israel, Cnidariophoma eilatica (incl. Cnidariophoma gen. nov.) from Stylophora pistillata. Italy, Lyophyllum obscurum on acidic soil. Namibia, Aureobasidium faidherbiae on dead leaf of Faidherbia albida, and Aureobasidium welwitschiae on dead leaves of Welwitschia mirabilis. Netherlands, Gaeumannomycella caricigena on dead culms of Carex elongata, Houtenomyces caricicola (incl. Houtenomyces gen. nov.) on culms of Carex disticha, Neodacampia ulmea (incl. Neodacampia gen. nov.) on branch of Ulmus laevis, Niesslia phragmiticola on dead standing culms of Phragmites australis, Pseudopyricularia caricicola on culms of Carex disticha, and Rhodoveronaea nieuwwulvenica on dead bamboo sticks. Norway, Arrhenia similis half-buried and moss-covered pieces of rotting wood in grass-grownpath. Pakistan, Mallocybe ahmadii on soil. Poland, Beskidomyces laricis (incl. Beskidomyces gen. nov.) from resin of Larix decidua ssp. polonica, Lapidomyces epipinicola from sooty mould community on Pinus nigra, and Leptographium granulatum from a gallery of Dendroctonus micans on Picea abies. Portugal, Geoglossum azoricum on mossy areas of laurel forest areas planted with Cryptomeria japonica, and Lunasporangiospora lusitanica from a biofilm covering a bio deteriorated limestone wall. Qatar, Alternaria halotolerans from hypersaline sea water, and Alternaria qatarensis from water sample collected from hypersaline lagoon. South Africa, Alfaria thamnochorti on culm of Thamnochortus fraternus, Knufia aloeicola on Aloe gariepensis, Muriseptatomyces restionacearum (incl.Muriseptatomyces gen. nov.) on culms of Restionaceae, Neocladosporium arctotis on nest of cases of bagworm moths(Lepidoptera, Psychidae) on Arctotis auriculata, Neodevriesia scadoxi on leaves of Scadoxus puniceus, Paraloratospora schoenoplecti on stems of Schoenoplectus lacustris, Tulasnella epidendrea from the roots of Epidendrum and Xenoidriella cinnamomi (incl. Xenoidriella gen. nov.) on leaf of Cinnamomum camphora. South Korea, Lemonniera fraxinea on decaying leaves of Fraxinus sp. frompond. Spain, Atheniella lauri on the bark of fallen trees of Laurus nobilis, Halocryptovalsa endophytica from surface-sterilised, asymptomatic roots of Salicornia patula, Inocybe amygdaliolens on soil in mixed forest, Inocybe pityusarum on calcareous soil in mixed forest, Inocybe roseobulbipes on acidic soils, Neonectria borealis from roots of Vitis berlandieri Sympoventuria eucalyptorum on leaves of Eucalyptus sp., and Tuber conchae fromsoil. Sweden, Inocybe bidumensis on calcareous soil. Thailand, Cordyceps sandindaengensis on Lepidoptera pupa, buried in soil, Ophiocordyceps kuchinaraiensis on Coleoptera larva, buried in soil, and Samsoniella winandae on Lepidoptera pupa, buriedinsoil. Taiwan region (China), Neophaeosphaeria livistonae on dead leaf of Livistona rotundifolia. Türkiye, Melanogaster anatolicus on clay loamy soils. UK, Basingstokeomyces allii (incl. Basingstokeomyces gen. nov.) on leaves of Allium schoenoprasum. Ukraine, Xenosphaeropsis corni on recently dead stem of Cornus alba. USA, Nothotrichosporon aquaticum (incl. Nothotrichosporon gen. nov.) from water, and Periconia philadelphiana from swab of coil surface. Morphological and culture characteristics for these new taxa are supported by DNA barcodes.
    https://doi.org/doi:10.3767/persoonia.2023.50.05
  • IMA Fungus
    30-03-2023

    Polydomus karssenii gen. nov. sp. nov. is a dark septate endophyte with a bifunctional lifestyle parasitising eggs of plant parasitic cyst nematodes (Heterodera spp.)

    Samad Ashrafi, Jan Peer Wennrich, Yvonne Becker, Jose Maciá Vicente, Anke Brißke-Rode, Matthias Daub, Torsten Thünen, Abdelfattah A. Dababat, Maria R. Finckh, Marc Stadler, Wolfgang Maier

    In this study fungal strains were investigated, which had been isolated from eggs of the cereal cyst nematode Heterodera filipjevi, and roots of Microthlaspi perfoliatum (Brassicaceae). The morphology, the interaction with nematodes and plants and the phylogenetic relationships of these strains originating from a broad geographic range covering Western Europe to Asia Minor were studied. Phylogenetic analyses using five genomic loci including ITSrDNA, LSUrDNA, SSUrDNA, rpb2 and tef1-α were carried out. The strains were found to represent a distinct phylogenetic lineage most closely related to Equiseticola and Ophiosphaerella, and Polydomus karssenii (Phaeosphaeriaceae, Pleosporales) is introduced here as a new species representing a monotypic genus. The pathogenicity tests against nematode eggs fulfilled Koch’s postulates using in vitro nematode bioassays and showed that the fungus could parasitise its original nematode host H. filipjevi as well as the sugar beet cyst nematode H. schachtii, and colonise cysts and eggs of its hosts by forming highly melanised moniliform hyphae. Light microscopic observations on fungus-root interactions in an axenic system revealed the capacity of the same fungal strain to colonise the roots of wheat and produce melanised hyphae and microsclerotia-like structure typical for dark septate endophytes. Confocal laser scanning microscopy further demonstrated that the fungus colonised the root cells by predominant intercellular growth of hyphae, and frequent formation of appressorium-like as well as penetration peg-like structures through internal cell walls surrounded by callosic papilla-like structures. Different strains of the new fungus produced a nearly identical set of secondary metabolites with various biological activities including nematicidal effects irrespective of their origin from plants or nematodes.

    https://doi.org/10.1186/s43008-023-00113-w
  • Journal of Ecology
    12-2022

    Deciphering the interactions between plant species and their main fungal root pathogens in mixed grassland communities

    Eline Ampt, Davide Francioli, Jasper van Ruijven, Sofia I. F. Gomes, Jose Maciá Vicente, Aad J Termorshuizen, Lisette M. Bakker, Liesje Mommer
    https://doi.org/10.1111/1365-2745.14012
  • Global Change Biology
    11-2022

    Global patterns in endemicity and vulnerability of soil fungi

    Leho Tedersoo, Vladimir S. Mikryukov, Alexander Zizka, Mohammad Bahram, Niloufar Hagh-Doust, Sten Anslan, Oleh Prylutskyi, Manuel Delgado-Baquerizo, Fernando T. Maestre, Jaan Pärn, Maarja Öpik, Mari Moora, Martin Zobel, Mikk Espenberg, Ülo Mander, Abdul Nasir Khalid, Adriana Corrales, Ahto Agan, Aida Marcela Vasco-Palacios, Alessandro Saitta, Andrea Rinaldi, Annemieke Verbeken, Bobby P. Sulistyo, Boris Tamgnoue, Brendan Furneaux, Camila Duarte Ritter, Casper Nyamukondiwa, Cathy Sharp, César Marín, Daniyal Gohar, Darta Klavina, Dipon Sharmah, Dong-Qin Dai, Eduardo Nouhra, Elisabeth Machteld Biersma, Elisabeth Rähn, Erin K. Cameron, Eske De Crop, Eveli Otsing, Evgeny A. Davydov, Felipe E. Albornoz, Francis Q. Brearley, Franz Buegger, Geoffrey Zahn, Gregory Bonito, Inga Hiiesalu, Isabel C. Barrio, Jacob Heilmann-Clausen, Jelena Ankuda, John Y. Kupagme, Jose Maciá Vicente, Joseph Djeugap Fovo, József Geml, Juha M. Alatalo, Julieta Alvarez-Manjarrez, Kadri Põldmaa, Kadri Runnel, Kalev Adamson, Kari Anne Bråthen, Karin Pritsch, Kassim I. Tchan, Kęstutis Armolaitis, Kevin D. Hyde, Kevin K. Newsham, Kristel Panksep, Adebola Azeez Lateef, Liis Tiirmann, Linda Hansson, Louis J. Lamit, Malka Saba, Maria Tuomi, Marieka Gryzenhout, Marijn Bauters, Meike Piepenbring, Nalin N. Wijayawardene, Nourou S. Yorou, Olavi Kurina, Peter E. Mortimer, Peter Meidl, Petr Kohout, R. Henrik Nilsson, Rasmus Puusepp, Rein Drenkhan, Roberto Garibay-Orijel, Roberto Godoy, Saad Alkahtani, Saleh Rahimlou, Sergey V. Dudov, Sergei Põlme, Soumya Ghosh, Sunil Mundra, Talaat Ahmed, Tarquin Netherway, Terry W. Henkel, Tomas L. Roslin, Vincent Nteziryayo, Vladimir E. Fedosov, Vladimir G. Onipchenko, W. A. Erandi Yasanthika, Young Woon Lim, Nadejda A. Soudzilovskaia, Alexandre Antonelli, Urmas Kõljalg, Kessy Abarenkov

    Fungi are highly diverse organisms, which provide multiple ecosystem services. However, compared with charismatic animals and plants, the distribution patterns and conservation needs of fungi have been little explored. Here, we examined endemicity patterns, global change vulnerability and conservation priority areas for functional groups of soil fungi based on six global surveys using a high-resolution, long-read metabarcoding approach. We found that the endemicity of all fungi and most functional groups peaks in tropical habitats, including Amazonia, Yucatan, West-Central Africa, Sri Lanka, and New Caledonia, with a negligible island effect compared with plants and animals. We also found that fungi are predominantly vulnerable to drought, heat and land-cover change, particularly in dry tropical regions with high human population density. Fungal conservation areas of highest priority include herbaceous wetlands, tropical forests, and woodlands. We stress that more attention should be focused on the conservation of fungi, especially root symbiotic arbuscular mycorrhizal and ectomycorrhizal fungi in tropical regions as well as unicellular early-diverging groups and macrofungi in general. Given the low overlap between the endemicity of fungi and macroorganisms, but high conservation needs in both groups, detailed analyses on distribution and conservation requirements are warranted for other microorganisms and soil organisms.

    https://doi.org/10.1111/gcb.16398
  • mSystems
    28-06-2022

    Nutrient Availability Does Not Affect Community Assembly in Root-Associated Fungi but Determines Fungal Effects on Plant Growth

    Jose Maciá Vicente, Bing Bai, Run Qi, Sebastian Ploch, Florian Breider, Marco Thines
    ABSTRACTNonmycorrhizal root-colonizing fungi are key determinants of plant growth, driving processes ranging from pathogenesis to stress alleviation. Evidence suggests that they might also facilitate host access to soil nutrients in a mycorrhiza-like manner, but the extent of their direct contribution to plant nutrition is unknown. To study how widespread such capacity is across root-colonizing fungi, we surveyed soils in nutrient-limiting habitats using plant baits to look for fungal community changes in response to nutrient conditions. We established a fungal culture collection and used Arabidopsis thaliana inoculation bioassays to assess the ability of fungi to facilitate host’s growth in the presence of organic nutrients unavailable to plants. Plant baits captured a representation of fungal communities extant in natural habitats and showed that nutrient limitation has little influence on community assembly. Arabidopsis thaliana inoculated with 31 phylogenetically diverse fungi exhibited a consistent fungus-driven growth promotion when supplied with organic nutrients compared to untreated plants. However, direct phosphorus measurement and RNA-seq data did not support enhanced nutrient uptake but rather that growth effects may result from changes in the plant’s immune response to colonization. The widespread and consistent host responses to fungal colonization suggest that distinct, locally adapted nonmycorrhizal fungi affect plant performance across habitats.
    IMPORTANCE Recent studies have shown that root-associated fungi that do not engage in classical mycorrhizal associations can facilitate the hosts’ access to nutrients in a mycorrhiza-like manner. However, the generality of this capacity remains to be tested. Root-associated fungi are frequently deemed major determinants of plant diversity and performance, but in the vast majority of cases their ecological roles in nature remain unknown. Assessing how these plant symbionts affect plant productivity, diversity, and fitness is important to understanding how plant communities function. Recent years have seen important advances in the understanding of the main drivers of the diversity and structure of plant microbiomes, but a major challenge is still linking community properties with function. This study contributes to the understanding of the cryptic function of root-associated fungi by testing their ability to participate in a specific process: nutrient acquisition by plants.
    https://doi.org/10.1128/msystems.00304-22
  • Persoonia
    06-2022

    Fungal Planet description sheets: 1383-1435

    Pedro W. Crous, Susanne J Boers, D.G. Holdom, E.R. Osieck, T.V. Steinrucken, Y.P. Tan, J.S. Vitelli, Roger G. Shivas, M.D. Barrett, G.A. Boxshall, J. Broadbridge, E. Larsson, T. Lebel, U. Pinruan, S. Sommai, P. Alvarado, G. Bonito, C.A. Decock, S. De la Peña-Lastra, G. Delgado, Jos Houbraken, Jose Maciá Vicente, Huzefa A. Raja, A. Rigueiro-Rodríguez, A. Rodrıguez, Michael J. Wingfield, S.J. Adams, A. Akulov, T. AL-Hidmi, V. Antonın, S. Arauzo, F. Arenas, F. Armada, J. Aylward, J.-M. Bellanger, A. Berraf-Tebbal, A. Bidaud, F. Boccardo, J. Cabero, F. Calledda, Gilles Corriol, J.L. Crane, J.D.W. Dearnaley, B. Dima, F. Dovana, A. Eichmeier, F. Esteve-Raventos, M. Fine, L. Ganzert, D. Garcıa, D. Torres-Garcia, J. Gene, A. Gutierrez, P. Iglesias, Ł. Istel, Panrada Jangsantear, G.M. Jansen, Mikael Jeppson, N.C. Karun, A. Karich, P. Khamsuntorn, K. Kokkonen, M. Kolarık, A. Kubatova, R Labuda, A.C. Lagashetti, N. Lifshitz, Celeste C. Linde, M. Loizides, Janet Jennifer Luangsa-ard, P. Lueangjaroenkit, S. Mahadevakumar, A.E. Mahamedi, D.W. Malloch, S. Marincowitz, A. Mateos, P.-A. Moreau, Andrew N. Miller, A. Molia, A. Morte, A. Navarro-Rodenas, J. Nebesaova, E. Nigrone, B.R. Nuthan, N H Oberlies, A.L. Pepori, T. Rämä, D. Rapley, K. Reschke, B.M. Robicheau, F. Roets, J. Roux, M. Saavedra, B. Sakolrak, A. Santini, H. evıkova, Paras Nath Singh, Sanjay K Singh, S. Somrithipol, M. Spetik, K.R. Sridhar, M. Starink-Willemse, V.A. Taylor, A.L. van Iperen, Jukka Vauras, A. K. Walker, Brenda D Wingfield, O. Yarden, A.W. Cooke, A.G. Manners, K.G. Pegg, Ewald Groenewald
    Novel species of fungi described in this study include those from various countries as follows: Australia, Agaricus albofoetidus, Agaricus aureoelephanti and Agaricus parviumbrus on soil, Fusarium ramsdenii from stem cankers of Araucaria cunninghamii, Keissleriella sporoboli from stem of Sporobolus natalensis, Leptosphaerulina queenslandica and Pestalotiopsis chiaroscuro from leaves of Sporobolus natalensis, Serendipita petricolae as endophyte from roots of Eriochilus petricola, Stagonospora tauntonensis from stem of Sporobolus natalensis, Teratosphaeria carnegiei from leaves of Eucalyptus grandis × E. camaldulensis and Wongia ficherai from roots of Eragrostis curvula. Canada, Lulworthia fundyensis from intertidal wood and Newbrunswickomyces abietophilus (incl. Newbrunswickomyces gen. nov.) on buds of Abies balsamea. Czech Republic, Geosmithia funiculosa from a bark beetle gallery on Ulmus minor and Neoherpotrichiella juglandicola (incl. Neoherpotrichiella gen. nov.) from wood of Juglans regia. France, Aspergillus rouenensis and Neoacrodontium gallica (incl. Neoacrodontium gen. nov.) from bore dust of Xestobium rufovillosum feeding on Quercus wood, Endoradiciella communis (incl. Endoradiciella gen. nov.) endophytic in roots of Microthlaspi perfoliatum and Entoloma simulans on soil. India, Amanita konajensis on soil and Keithomyces indicus from soil. Israel, Microascus rothbergiorum from Stylophora pistillata. Italy, Calonarius ligusticus on soil. Netherlands, Appendopyricularia juncicola (incl. Appendopyricularia gen. nov.), Eriospora juncicola and Tetraploa juncicola on dead culms of Juncus effusus, Gonatophragmium physciae on Physcia caesia and Paracosmospora physciae (incl. Paracosmospora gen. nov.) on Physcia tenella, Myrmecridium phragmitigenum on dead culm of Phragmites australis, Neochalara lolae on stems of Pteridium aquilinum, Niesslia nieuwwulvenica on dead culm of undetermined Poaceae, Nothodevriesia narthecii (incl. Nothodevriesia gen. nov.) on dead leaves of Narthecium ossifragum and Parastenospora pini (incl. Parastenospora gen. nov.) on dead twigs of Pinus sylvestris. Norway, Verticillium bjoernoeyanum from sand grains attached to a piece of driftwood on a sandy beach. Portugal, Collybiopsis cimrmanii on the base of living Quercus ilex and amongst dead leaves of Laurus and herbs. South Africa, Paraproliferophorum hyphaenes (incl. Paraproliferophorum gen. nov.) on living leaves of Hyphaene sp. and Saccothecium widdringtoniae on twigs of Widdringtonia wallichii. Spain, Cortinarius dryosalor on soil, Cyphellophora endoradicis endophytic in roots of Microthlaspi perfoliatum, Geoglossum laurisilvae on soil, Leptographium gemmatum from fluvial sediments, Physalacria auricularioides from a dead twig of Castanea sativa, Terfezia bertae and Tuber davidlopezii in soil. Sweden, Alpova larskersii, Inocybe alpestris and Inocybe boreogodeyi on soil. Thailand, Russula banwatchanensis, Russula purpureoviridis and Russula lilacina on soil. Ukraine, Nectriella adonidis on overwintered stems of Adonis vernalis. USA, Microcyclus jacquiniae from living leaves of Jacquinia keyensis and Penicillium neoherquei from a minute mushroom sporocarp. Morphological and culture characteristics are supported by DNA barcodes.
    https://doi.org/10.3767/persoonia.2022.48.08
  • CZECH MYCOLOGY
    15-03-2022

    Leptodophora gen. nov. (Helotiales, Leotiomycetes) proposed to accommodate selected root-associated members of the genus Cadophora

    Ondřej Koukol, Jose Maciá Vicente
    Cadophora orchidicola, a root-associated asexual species, did not have a clear placement within the paraphyletic genus Cadophora because it was not represented by sequences derived from the type material. Based on four newly obtained molecular marker sequences from the ex-type strain, a new genus, Leptodophora, is proposed, typified with C. orchidicola, as well as new combinations for three recently described and closely related species. In addition, a new species, Collembolispora disimilis, is proposed for a fungus producing phialidic conidiogenous cells and previously treated as Cadophora sp.
    https://doi.org/10.33585/cmy.74104
  • 03-2022

    Towards understanding diversity, endemicity and global change vulnerability of soil fungi

    L. Tedersoo, Vladimir S. Mikryukov, Alexander Zizka, Mohammad Bahram, Niloufar Hagh-Doust, Sten Anslan, Oleh Prylutskyi, Manuel Delgado-Baquerizo, Fernando T. Maestre, Jaan Pärn, Maarja Öpik, Mari Moora, Martin Zobel, Kessy Abarenkov, Jose Maciá Vicente
    https://doi.org/10.1101/2022.03.17.484796
  • Persoonia
    2022

    Fungal Planet description sheets: 1436–1477

    Y.P. Tan, S.L. Bishop-Hurley, Roger G. Shivas, D.A. Cowan, G. Maggs-Kölling, Sajeewa S. N. Maharachchikumbura, U. Pinruan, K.L. Bransgrove, S. De la Peña-Lastra, E. Larsson, T. Lebel, S. Mahadevakumar, A. Mateos, E.R. Osieck, A. Rigueiro-Rodríguez, S. Sommai, K. Ajithkumar, A. Akulov, Freda E. Anderson, F. Arenas, S. Balashov, Á. Bañares, D.K. Berger, M. Virginia Bianchinotti, S. Bien, P. Bilański, G.A. Boxshall, M. Bradshaw, J. Broadbridge, F.J.S. Calaça, C. Campos-Quiroz, J. Carrasco-Fernández, J.F. Castro, S. Chaimongkol, S. Chandranayaka, Y. Chen, D. Comben, J.D.W. Dearnaley, A.S. Ferreira-Sá, K. Dhileepan, M.L. Díaz, Pradeep K. Divakar, S. Xavier-Santos, A. Fernández-Bravo, J. Gené, F.E. Guard, M. Diaz-Guerra, S. Gunaseelan, Jos Houbraken, K. Janik-Superson, R. Jankowiak, Mikael Jeppson, Ž. Jurjević, M. Kaliyaperumal, L.A. Kelly, K. Kezo, Abdul Nasir Khalid, P. Khamsuntorn, D. Kidanemariam, M. Kiran, E. Lacey, G.J. Langer, L.V. López-Llorca, Janet Jennifer Luangsa-ard, P. Lueangjaroenkit, H. Thorsten Lumbsch, Jose Maciá Vicente, L.S. Mamatha Bhanu, T.S. Marney, J.E. Marqués-Gálvez, A. Morte, A. Naseer, A. Navarro-Ródenas, Oluwawapelumi A. Oyedele, S. A. Peters, S. Piskorski, Luis Quijada, G.H. Ramírez, K. Raja, A. Razzaq, Víctor J Rico, Asun Rodríguez-Uña, M. Ruszkiewicz-Michalska, R.M. Sánchez, C. Santelices, A.S Savitha, Manuel Serrano, L. Leonardo-Silva, H. Solheim, S. Somrithipol, M.Y. Sreenivasa, H. Stępniewska, D. Strapagiel, Amaro Taylor-Weiner, D. Torres-Garcia, Jukka Vauras, M. Villarreal, Cobus Visagie, M. Wołkowycki, W. Yingkunchao, E. Zapora, Ewald Groenewald, Pedro W. Crous
    Novel species of fungi described in this study include those from various countries as follows: Argentina, Colletotrichum araujiae on leaves, stems and fruits of Araujia hortorum. Australia, Agaricus pateritonsus on soil, Curvularia fraserae on dying leaf of Bothriochloa insculpta, Curvularia millisiae from yellowing leaf tips of Cyperus aromaticus, Marasmius brunneolorobustus on well-rotted wood, Nigrospora cooperae from necrotic leaf of Heteropogon contortus, Penicillium tealii from the body of a dead spider, Pseudocercospora robertsiorum from leaf spots of Senna tora, Talaromyces atkinsoniae from gills of Marasmius crinis-equi and Zasmidium pearceae from leaf spots of Smilax glyciphylla. Brazil, Preussia bezerrensis from air. Chile, Paraconiothyrium kelleni from the rhizosphere of Fragaria chiloensis subsp. chiloensis f. chiloensis. Finland, Inocybe udicola on soil in mixed forest with Betula pendula, Populus tremula, Picea abies and Alnus incana. France, Myrmecridium normannianum on dead culm of unidentified Poaceae. Germany, Vexillomyces fraxinicola from symptomless stem wood of Fraxinus excelsior. India, Diaporthe limoniae on infected fruit of Limonia acidissima, Didymella naikii on leaves of Cajanus cajan, and Fulvifomes mangroviensis on basal trunk of Aegiceras corniculatum. Indonesia, Penicillium ezekielii from Zea mays kernels. Namibia, Neocamarosporium calicoremae and Neocladosporium calicoremae on stems of Calicorema capitata, and Pleiochaeta adenolobi on symptomatic leaves of Adenolobus pechuelii. Netherlands, Chalara pteridii on stems of Pteridium aquilinum, Neomackenziella juncicola (incl. Neomackenziella gen. nov.) and Sporidesmiella junci from dead culms of Juncus effusus. Pakistan, Inocybe longistipitata on soil in a Quercus forest. Poland, Phytophthora viadrina from rhizosphere soil of Quercus robur, and Septoria krystynae on leaf spots of Viscum album. Portugal (Azores), Acrogenospora stellata on dead wood or bark. South Africa, Phyllactinia greyiae on leaves of Greyia sutherlandii and Punctelia anae on bark of Vachellia karroo. Spain, Anteaglonium lusitanicum on decaying wood of Prunus lusitanica subsp. lusitanica, Hawksworthiomyces riparius from fluvial sediments, Lophiostoma carabassense endophytic in roots of Limbarda crithmoides, and Tuber mohedanoi from calcareus soils. Spain (Canary Islands), Mycena laurisilvae on stumps and woody debris. Sweden, Elaphomyces geminus from soil under Quercus robur. Thailand, Lactifluus chiangraiensis on soil under Pinus merkusii, Lactifluus nakhonphanomensis and Xerocomus sisongkhramensis on soil under Dipterocarpus trees. Ukraine, Valsonectria robiniae on dead twigs of Robinia hispida. USA, Spiralomyces americanus (incl. Spiralomyces gen. nov.) from office air. Morphological and culture characteristics are supported by DNA barcodes.
    https://doi.org/10.3767/persoonia.2022.49.08
  • Fungal Diversity
    11-2021

    The Global Soil Mycobiome consortium dataset for boosting fungal diversity research

    Leho Tedersoo, Vladimir S. Mikryukov, Sten Anslan, Mohammad Bahram, Abdul Nasir Khalid, Adriana Corrales, Ahto Agan, Aida Marcela Vasco-Palacios, Alessandro Saitta, Alexandre Antonelli, Andrea Rinaldi, Annemieke Verbeken, Bobby P. Sulistyo, Boris Tamgnoue, Brendan Furneaux, Camila Duarte Ritter, Casper Nyamukondiwa, Cathy Sharp, César Marín, Dong-Qin Dai, Daniyal Gohar, Dipon Sharmah, Elisabeth Machteld Biersma, Erin K. Cameron, Eske De Crop, Eveli Otsing, Evgeny A. Davydov, Felipe E. Albornoz, Francis Q. Brearley, Franz Buegger, Genevieve Gates, Geoffrey Zahn, Gregory Bonito, Indrek Hiiesalu, Inga Hiiesalu, Irma Zettur, Isabel C. Barrio, Jaan Pärn, Jacob Heilmann-Clausen, Jelena Ankuda, John Y. Kupagme, Joosep Sarapuu, Jose Maciá Vicente, Joseph Djeugap Fovo, József Geml, Juha M. Alatalo, Julieta Alvarez-Manjarrez, Jutamart Monkai, Kadri Põldmaa, Kadri Runnel, Kalev Adamson, Kari Anne Bråthen, Karin Pritsch, Kassim I. Tchan, Kęstutis Armolaitis, Kevin D. Hyde, Kevin K. Newsham, Kristel Panksep, Adebola Azeez Lateef, Louis J. Lamit, Malka Saba, Marcela E. da Silva Cáceres, Maria Tuomi, Marieka Gryzenhout, Marijn Bauters, Miklós Bálint, Nalin N. Wijayawardene, Niloufar Hagh-Doust, Nourou S. Yorou, Olavi Kurina, Peter E. Mortimer, Peter Meidl, R. Henrik Nilsson, Rasmus Puusepp, Rebeca Casique-Valdés, Rein Drenkhan, Roberto Garibay-Orijel, Roberto Godoy, Saleh Alfarraj, Saleh Rahimlou, Sergei Põlme, Sergey V. Dudov, Sunil Mundra, Talaat Ahmed, Tarquin Netherway, Terry W. Henkel, Tomas L. Roslin, Vladimir E. Fedosov, Vladimir G. Onipchenko, W. A. Erandi Yasanthika, Young Woon Lim, Meike Piepenbring, Darta Klavina, Urmas Kõljalg, Kessy Abarenkov

    Fungi are highly important biotic components of terrestrial ecosystems, but we still have a very limited understanding about their diversity and distribution. This data article releases a global soil fungal dataset of the Global Soil Mycobiome consortium (GSMc) to boost further research in fungal diversity, biogeography and macroecology. The dataset comprises 722,682 fungal operational taxonomic units (OTUs) derived from PacBio sequencing of full-length ITS and 18S-V9 variable regions from 3200 plots in 108 countries on all continents. The plots are supplied with geographical and edaphic metadata. The OTUs are taxonomically and functionally assigned to guilds and other functional groups. The entire dataset has been corrected by excluding chimeras, index-switch artefacts and potential contamination. The dataset is more inclusive in terms of geographical breadth and phylogenetic diversity of fungi than previously published data. The GSMc dataset is available over the PlutoF repository.

    https://doi.org/10.1007/s13225-021-00493-7
  • Persoonia
    06-2021

    Fungal Planet description sheets: 1182–1283

    Pedro W. Crous, D.A. Cowan, G. Maggs-Kölling, N. Yilmaz, R. Thangavel, Michael J. Wingfield, Machiel E Noordeloos, B. Dima, T.E. Brandrud, G.M. Jansen, O V Morozova, J. Vila, Roger G. Shivas, Y.P. Tan, S. Bishop‐Hurley, E. Lacey, T.S. Marney, E. Larsson, G. Le Floch, Lorenzo Lombard, P. Nodet, V. Hubka, P. Alvarado, A. Berraf-Tebbal, Juan de Dios Reyes García, G. Delgado, A. Eichmeier, J.B. Jordal, A V Kachalkin, A. Kubátová, Jose Maciá Vicente, E.F. Malysheva, V. Papp, Kunhiraman C. Rajeshkumar, A. Sharma, M. Spetik, D. Szabóová, M.A. Tomashevskaya, J.A. Abad, Z.G. Abad, A V Alexandrova, G. Anand, F. Arenas, N. Ashtekar, S. Balashov, Á. Bañares, R. Baroncelli, I. Bera, Alona Yu Biketova, C.L. Blomquist, Teun Boekhout, David Boertmann, T.M. Bulyonkova, T.I. Burgess, A.J. Carnegie, J.F. Cobo‐Diaz, Gilles Corriol, J.H. Cunnington, M.O. da Cruz, U. Damm, N. Davoodian, A.L.C.M. de A. Santiago, John Dearnaley, L.W.S. de Freitas, K. Dhileepan, R A Dimitrov, Silvano Piazza, S. Fatima, F. Fuljer, H. Galera, A. Ghosh, A. Giraldo, A.M. Glushakova, M. Gorczak, D E Gouliamova, D. Gramaje, Marizeth Groenewald, C.K. Gunsch, A. Gutiérrez, D.G. Holdom, Jos Houbraken, A.B. Ismailov, Ł. Istel, T. Iturriaga, Mikael Jeppson, Ž. Jurjević, Liudmila B Kalinina, V.I. Kapitonov, I. Kautmanova, Abdul Nasir Khalid, M. Kiran, L. Kiss, Á. Kovács, D. Kurose, I Kusan, S.S. Lad, Thomas Læssøe, H.B. Lee, Janet Jennifer Luangsa-ard, Joshua M Lynch, A.E. Mahamedi, V.F. Malysheva, A. Mateos, N. Matočec, A. Mešić, Andrew N. Miller, S. Mongkolsamrit, G. Moreno, A. Morte, R. Mostowfizadeh‐Ghalamfarsa, A. Naseer, A. Navarro-Ródenas, Thuong T. T. Nguyen, W. Noisripoom, J.E. Ntandu, Jorinde Nuytinck, V. Ostrý, T.A. Pankratov, J. Pawłowska, J. Pecenka, T.H.G. Pham, A. Polhorský, A. Posta, D.B. Raudabaugh, K. Reschke, Víctor Arroyo-Rodríguez, Ana M Millanes Romero, S. Rooney-Latham, J. Roux, M. Sandoval-Denis, Maudy Smith, T.V. Steinrucken, Tatyana Yu Svetasheva, Z. Tkalčec, Elna J van der Linde, M. v.d. Vegte, Jukka Vauras, A. Verbeken, C.M. Visagie, J.S. Vitelli, S.V. Volobuev, A. Weill, M. Wrzosek, I.V. Zmitrovich, E.A. Zvyagina, Ewald Groenewald
    Novel species of fungi described in this study include those from various countries as follows: Algeria, Phaeoacremonium adelophialidum from Vitis vinifera. Antarctica, Comoclathris antarctica from soil. Australia, Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia, Eremothecium peggii in fruit of Citrus australis, Microdochium ratticaudae from stem of Sporobolus natalensis, Neocelosporium corymbiae on stems of Corymbia variegata, Phytophthora kelmanii from rhizosphere soil of Ptilotus pyramidatus, Pseudosydowia backhousiae on living leaves of Backhousia citriodora, Pseudosydowia indoor oopillyensis, Pseudosydowia louisecottisiae and Pseudosydowia queenslandica on living leaves of Eucalyptus sp. Brazil, Absidia montepascoalis from soil. Chile, Ilyonectria zarorii from soil under Maytenus boaria. Costa Rica, Colletotrichum filicis from an unidentified fern. Croatia, Mollisia endogranulata on deteriorated hardwood. Czech Republic, Arcopilus navicularis from tea bag with fruit tea, Neosetophoma buxi as endophyte from Buxus sempervirens, Xerochrysium bohemicum on surface of biscuits with chocolate glaze and filled with jam. France, Entoloma cyaneobasale on basic to calcareous soil, Fusarium aconidiale from Triticum aestivum, Fusarium juglandicola from buds of Juglans regia. Germany, Tetraploa endophytica as endophyte from Microthlaspi perfoliatum roots. India, Castanediella ambae on leaves of Mangifera indica, Lactifluus kanadii on soil under Castanopsis sp., Penicillium uttarakhandense from soil. Italy, Penicillium ferraniaense from compost. Namibia, Bezerromyces gobabebensis on leaves of unidentified succulent, Cladosporium stipagrostidicola on leaves of Stipagrostis sp., Cymostachys euphorbiae on leaves of Euphorbia sp., Deniquelata hypolithi from hypolith under a rock, Hysterobrevium walvisbayicola on leaves of unidentified tree, Knufia hypolithi and Knufia walvisbayicola from hypolith under a rock, Lapidomyces stipagrostidicola on leaves of Stipagrostis sp., Nothophaeotheca mirabibensis (incl. Nothophaeotheca gen. nov.) on persistent inflorescence remains of Blepharis obmitrata, Paramyrothecium salvadorae on twigs of Salvadora persica, Preussia procaviicola on dung of Procavia sp., Sordaria equicola on zebra dung, Volutella salvadorae on stems of Salvadora persica. Netherlands, Entoloma ammophilum on sandy soil, Entoloma pseudocruentatum on nutrient poor(acid)soil, Entoloma pudens on plant debris, amongst grasses. New Zealand, Amorocoelophoma neoregeliae from leaf spots of Neoregelia sp., Aquilomyces metrosideri and Septoriella callistemonis from stem discolouration and leaf spots of Metrosideros sp., Cadophora neoregeliae from leaf spots of Neoregelia sp., Flexuomyces asteliae (incl. Flexuomyces gen. nov.) and Mollisia asteliae from leaf spots of Astelia chathamica, Ophioceras freycinetiae from leaf spots of Freycinetia banksii, Phaeosphaeria caricis-sectae from leaf spots of Carex secta. Norway, Cuphophyllus flavipesoides on soil in semi-natural grassland, Entoloma coracis on soil in calcareous Pinus and Tilia forests, Entoloma cyaneolilacinum on soil semi-natural grasslands, Inocybe norvegica on gravelly soil. Pakistan, Butyriboletus parachinarensis on soil in association with Quercus baloot. Poland, Hyalodendriella bialowiezensis on debris beneath fallen bark of Norway spruce Picea abies. Russia, Bolbitius sibiricus on a moss covered rotting trunk of Populus tremula, Crepidotus wasseri on debris of Populus tremula, Entoloma isborscanum on soil on calcareous grasslands, Entoloma subcoracis on soil in subalpine grasslands, Hydropus lecythiocystis on rotted wood of Betula pendula, Meruliopsis faginea on fallen dead branches of Fagus orientalis, Metschnikowia taurica from fruits of Ziziphus jujube, Suillus praetermissus on soil, Teunia lichenophila as endophyte from Cladonia rangiferina. Slovakia, Hygrocybe fulgens on mowed grassland, Pleuroflammula pannonica from corticated branches of Quercus sp. South Africa, Acrodontium burrowsianum on leaves of unidentified Poaceae, Castanediella senegaliae on dead pods of Senegalia ataxacantha, Cladophialophora behniae on leaves of Behnia sp., Colletotrichum cliviigenum on leaves of Clivia sp., Diatrype dalbergiae on bark of Dalbergia armata, Falcocladium heteropyxidicola on leaves of Heteropyxis canescens, Lapidomyces aloidendricola as epiphyte on brown stem of Aloidendron dichotomum, Lasionectria sansevieriae and Phaeosphaeriopsis sansevieriae on leaves of Sansevieria hyacinthoides, Lylea dalbergiae on Diatrype dalbergiae on bark of Dalbergia armata, Neochaetothyrina syzygii (incl. Neochaetothyrina gen. nov.) on leaves of Syzygium chordatum, Nothophaeomoniella ekebergiae (incl. Nothophaeomoniella gen. nov.) on leaves of Ekebergia pterophylla, Paracymostachys euphorbiae (incl. Paracymostachys gen. nov.)on leaf litter of Euphorbia ingens, Paramycosphaerella pterocarpi on leaves of Pterocarpus angolensis, Paramycosphaerella syzygii on leaf litter of Syzygium chordatum, Parateichospora phoenicicola (incl. Parateichospora gen. nov.)on leaves of Phoenix reclinata, Seiridium syzygii on twigs of Syzygium chordatum, Setophoma syzygii on leaves of Syzygium sp., Starmerella xylocopis from larval feed of an Afrotropical bee Xylocopa caffra, Teratosphaeria combreti on leaf litter of Combretum kraussii, Teratosphaericola leucadendri on leaves of Leucadendron sp., Toxicocladosporium pterocarpi on pods of Pterocarpus angolensis. Spain, Cortinarius bonachei with Quercus ilex in calcareus soils, Cortinarius brunneovolvatus under Quercus ilex subsp. ballota in calcareous soil, Extremopsis radicicola (incl. Extremopsis gen. nov.) from root-associated soil in a wet heathland, Russula quintanensis on acidic soils, Tubaria vulcanica on volcanic lapilii material, Tuber zambonelliae in calcareus soil. Sweden, Elaphomyces borealis on soil under Pinus sylvestris and Betula pubescens. Tanzania, Curvularia tanzanica on inflorescence of Cyperus aromaticus. Thailand, Simplicillium niveum on Ophiocordyceps camponoti-leonardi on under side of unidentified dicotyledonous leaf. USA, Calonectria californiensis on leaves of Umbellularia californica, Exophiala spartinae from surface sterilised roots of Spartina alterniflora, Neophaeococcomyces oklahomaensis from outside wall of alcohol distillery. Vietnam, Fistulinella aurantioflava on soil. Morphological and culture characteristics are supported by DNA barcodes.
    https://doi.org/10.3767/persoonia.2021.46.11
  • IMA Fungus
    12-2020

    Brassicaceous roots as an unexpected diversity hot-spot of helotialean endophytes

    Jose Maciá Vicente, Meike Piepenbring, Ondřej Koukol
    A high number of fungal strains were isolated from roots of Brassicaceae species collected across western and southern Europe, resulting in an unexpectedly rich collection of Cadophora species. These isolates enable us to present a new and comprehensive view of the ecological, morphological, and phylogenetic traits of root-inhabiting members of this helotialean genus. We provide phylogenetic placement of all of our isolates based on a four-gene dataset, analyze their phenotypic traits in relation to their phylogenetic relationships, and infer the potential distribution ranges of the species by sequence comparisons with available databases. We consider seven well supported phylogenetic lineages as species new to science. Six further lineages probably also represent new species but remain undescribed due to the lack of diagnostic morphological characters. Our results show that Cadophora, as currently circumscribed, is paraphyletic and encompasses a broad spectrum of morphologies and lifestyles. Among the new species, only two (C. ferruginea and C. constrictospora) form phialides and conidia typical of Cadophora, three species (C. echinata, C. gamsii and C. variabilis) produce chains of swollen hyphal segments that may function as holoblastic conidia, and one species (C. fascicularis) produces chains of holoblastic ramoconidia and conidia. Ancestral state reconstruction analysis suggests that phialidic conidiogenesis evolved several times in Cadophora s. lat. from a putatively holoblastic common ancestor. Most Cadophora lineages are rare as estimated from the availability of sequence data, in spite of having relatively wide distribution ranges, whereas five lineages may represent endemic relationships given their restricted distributions. Our dataset, probably the most comprehensive available for Cadophora, nevertheless shows knowledge gaps concerning the phylogenetic relationships within this genus and highlights a need for further investigation.
    https://doi.org/10.1186/s43008-020-00036-w
  • Mycological Progress
    01-10-2020

    Multilocus phylogeny- and fruiting feature-assisted delimitation of European Cyclocybe aegerita from a new Asian species complex and related species

    Roman A. Frings, Jose Maciá Vicente, Sandra Buße, Adéla Čmoková, Harald Kellner, Martin Hofrichter, Florian Hennicke

    Cyclocybe aegerita (synonym: Agrocybe aegerita) is a widely cultivated edible and reportedly almost cosmopolitan mushroom species that serves as a model fungus for basidiome formation and as producer of useful natural products and enzymes. Focusing on strains from different continents, here, we present a phylogenetic analysis of this species and some adjacent taxa that employs four phylogenetic markers. In addition, we tested the strains’ capability to fructify on agar media. Our analysis reveals that “C. aegerita sensu lato” splits up into the following two well-supported monophyletic geographic lineages: a European clade and an Asian clade. The European one is closely associated with the Chinese species Cyclocybe salicaceicola. In contrast, the Asian lineage, which we preliminarily designate as Cyclocybe chaxingu agg., may comprise several species (species complex) and clusters with the Pacific species Cyclocybe parasitica (New Zealand). In addition, fruiting properties differ across C. aegerita and its Asian and Pacific relatives; however, strains from the Asian clade and C. parasitica tend to form larger basidiomes with relatively big caps and long stipes and strains from the European clade exhibit a more variable fruiting productivity with the tendency to form more basidiomes, with smaller caps and shorter stipes. Moreover, some strains showed individual fruiting patterns, such as the preference to fruit where they were exposed to injuring stimuli. In conclusion, the delimitation of the newly delimited Asian species complex from our multilocus phylogeny of “C. aegerita sensu lato”, which is supported by phenotypic data, depicts an exemplary case of biogeographic diversity within a previously thought homogeneous species of near worldwide distribution.

    https://doi.org/10.1007/s11557-020-01599-z
  • Flora: Morphology, Distribution, Functional Ecology of Plants
    04-2019

    Out of Transcaucasia

    Tahir Ali, Violeta Muñoz-Fuentes, Ann Katrin Buch, Ali Çelik, Ayan Dutbayev, Ivan Gabrielyan, Kyriaki Glynou, Leila Kachour, Imran Khaliq, Miloslav Kitner, Lisa Nigrelli, Sebastian Ploch, Fabian Runge, Irina Solovyeva, Angelika Schmuker, Ljudmila Vakhrusheva, Xiaojuan Xia, Jose Maciá Vicente, Carsten Nowak, Marco Thines

    Quaternary climatic oscillations had strong effects on Palearctic biodiversity. Although Transcaucasia is hypothesised to be a centre of diversity for many taxa, relatively few studies have investigated its potential role as such, or as a Pleistocene refugium. Using AFLP and sequence data (ITS, matK, trnL-F) from samples collected across the entire range of Microthlaspi perfoliatum, from the Iberian peninsula to Kazakhstan, and including the Mediterranean Basin and Central Europe, in this study it is examined how the interplay of Pleistocene climatic oscillations and historical demography may have shaped the current genetic structure of the species. The results of this study provide evidence that M. perfoliatum survived Pleistocene glaciation in five major refugia, Iberia, southwestern France, Italy, the Balkans and Anatolia/Transcaucasia and a sixth, unknown refugium in the disjunct distribution area in Central Asia. Our analyses support an ancient colonisation of M. perfoliatum towards the western and central Palearctic, perhaps, during the early Pleistocene or late Pliocene, likely starting from Transcaucasia. However, postglacial recolonisation of western and Central Europe has taken place from two distinct refugia, namely southwestern France/northern Italy, and Anatolia/Southern Balkans, respectively. The Iberian populations apparently did not contribute to postglacial recolonisation of Europe, perhaps because the Pyrenees acted as a significant barrier to dispersal. It is also conceivable that a rapidly expanding population from southwestern France and the southeastern part of the range effectively blocked the establishment of latecomers. The present study thus reveals a complex pattern of colonisation of Europe from Transcaucasia, with an interplay of climate and physical geography as main factors shaping the present-day genetic structure of M. perfoliatum.

    https://doi.org/10.1016/j.flora.2019.02.012
  • Mycologia
    04-03-2018

    Inhabiting plant roots, nematodes, and truffles—Polyphilus, a new helotialean genus with two globally distributed species

    Samad Ashrafi, Dániel G Knapp, Damien Blaudez, Michel Chalot, Jose Maciá Vicente, Imre Zagyva, Abdelfattah A. Dababat, Wolfgang Maier, Gábor M Kovács
    https://doi.org/10.1080/00275514.2018.1448167
  • Environmental Microbiology
    03-2018

    Metabolomics‐based chemotaxonomy of root endophytic fungi for natural products discovery

    Jose Maciá Vicente, Yan‐Ni Shi, Zakaria Cheikh‐Ali, Peter Grün, Kyriaki Glynou, Sevda Haghi Kia, Meike Piepenbring, Helge B Bode
    https://doi.org/10.1111/1462-2920.14072
  • New Phytologist
    01-02-2018

    Facultative root-colonizing fungi dominate endophytic assemblages in roots of nonmycorrhizal Microthlaspi species

    Kyriaki Glynou, Bora Nam, Marco Thines, Jose Maciá Vicente

    There is increasing knowledge on the diversity of root-endophytic fungi, but limited information on their lifestyles and dependence on hosts hampers our understanding of their ecological functions. We compared diversity and biogeographical patterns of cultivable and noncultivable root endophytes to assess whether their occurrence is determined by distinct ecological factors. The endophytic diversity in roots of nonmycorrhizal Microthlaspi spp. growing across Europe was assessed using high-throughput sequencing (HTS) and compared with a previous dataset based on cultivation of endophytes from the same root samples. HTS revealed a large fungal richness undetected by cultivation, but which largely comprised taxa with restricted distributions and/or low representation of sequence reads. Both datasets coincided in a consistent high representation of widespread endophytes within orders Pleosporales, Hypocreales and Helotiales, as well as similar associations of community structure with spatial and environmental conditions. Likewise, distributions of particular endophytes inferred by HTS agreed with cultivation data in suggesting individual ecological preferences. Our findings support that Microthlaspi spp. roots are colonized mostly by saprotrophic and likely facultative endophytes, and that differential niche preferences and distribution ranges among fungi importantly drive the assembly of root-endophytic communities.

    https://doi.org/10.1111/nph.14873
  • FEMS Microbiology Ecology
    01-02-2018

    The effects of fungal root endophytes on plant growth are stable along gradients of abiotic habitat conditions

    Sevda Haghi Kia, Miroslava Jurkechova, Kyriaki Glynou, Meike Piepenbring, Jose Maciá Vicente
    https://doi.org/10.1093/femsec/fix162
  • Studies in Mycology
    02-2018

    Temporal variation of fungal diversity in a mosaic landscape in Germany

    Sebastian Rudolph, Jose Maciá Vicente, H. Lotz-Winter, M. Schleuning, Meike Piepenbring
    https://doi.org/10.1016/j.simyco.2018.01.001
  • Molecular Ecology
    09-2017

    Genotypic diversity in root-endophytic fungi reflects efficient dispersal and environmental adaptation

    Kyriaki Glynou, Tahir Ali, Sevda Haghi Kia, Marco Thines, Jose Maciá Vicente

    Studying community structure and dynamics of plant-associated fungi is the basis for unravelling their interactions with hosts and ecosystem functions. A recent sampling revealed that only a few fungal groups, as defined by internal transcribed spacer region (ITS) sequence similarity, dominate culturable root endophytic communities of nonmycorrhizal Microthlaspi spp. plants across Europe. Strains of these fungi display a broad phenotypic and functional diversity, which suggests a genetic variability masked by ITS clustering into operational taxonomic units (OTUs). The aims of this study were to identify how genetic similarity patterns of these fungi change across environments and to evaluate their ability to disperse and adapt to ecological conditions. A first ITS-based haplotype analysis of ten widespread OTUs mostly showed a low to moderate genotypic differentiation, with the exception of a group identified as Cadophora sp. that was highly diverse. A multilocus phylogeny based on additional genetic loci (partial translation elongation factor 1α, beta-tubulin and actin) and amplified fragment length polymorphism profiling of 185 strains representative of the five dominant OTUs revealed a weak association of genetic differences with geography and environmental conditions, including bioclimatic and soil factors. Our findings suggest that dominant culturable root endophytic fungi have efficient dispersal capabilities, and that their distribution is little affected by environmental filtering. Other processes, such as inter- and intraspecific biotic interactions, may be more important for the local assembly of their communities.

    https://doi.org/10.1111/mec.14231
  • Revista Iberoamericana de Micologia
    04-2017

    Hongos endófitos asociados con raíces de palmera datilera (Phoenix dactylifera) en dunas costeras

    Fadila Mohamed Mahmoud, Zoulikha Krimi, Jose Maciá Vicente, Mohamed Brahim Errahmani, Luis V. Lopez-Llorca

    Background Symbiotic interactions with fungal endophytes are argued to be responsible for the tolerance of plants to some stresses and for their adaptation to natural conditions. Aims In this study we aimed to examine the endophytic fungal diversity associated with roots of date palms growing in coastal dune systems, and to screen this collection of endophytes for potential use as biocontrol agents, for antagonistic activity and mycoparasitism, and as producers of antifungal compounds with potential efficacy against root diseases of date palm. Methods Roots of nine individual date palms growing in three coastal locations in the South-East of Spain (Guardamar, El Carabassí, and San Juan) were selected to isolate endophytic fungi. Isolates were identified on the basis of morphological and/or molecular characters. Results Five hundred and fifty two endophytic fungi were isolated and assigned to thirty morphological taxa or molecular operational taxonomic units. Most isolates belonged to Ascomycota, and the dominant order was Hypocreales. Fusarium and Clonostachys were the most frequently isolated genera and were present at all sampling sites. Comparisons of the endophytic diversity with previous studies, and their importance in the management of the date palm crops are discussed. Conclusions This is the first study on the diversity of endophytic fungi associated with roots of date palm. The isolates obtained might constitute a source of biological control agents and biofertilizers for use in crops of this plant.

    https://doi.org/10.1016/j.riam.2016.06.007
  • ISME Journal
    01-03-2017

    Influence of phylogenetic conservatism and trait convergence on the interactions between fungal root endophytes and plants

    Sevda Haghi Kia, Kyriaki Glynou, Thomas Nau, Marco Thines, Meike Piepenbring, Jose Maciá Vicente

    Plants associate through their roots with fungal assemblages that impact their abundance and productivity. Non-mycorrhizal endophytes constitute an important component of such fungal diversity, but their implication in ecosystem processes is little known. Using a selection of 128 root-endophytic strains, we defined functional groups based on their traits and plant interactions with potential to predict community assembly and symbiotic association processes. In vitro tests of the strains' interactions with Arabidopsis thaliana, Microthlaspi erraticum and Hordeum vulgare showed a net negative effect of fungal colonization on plant growth. The effects partly depended on the phylogenetic affiliation of strains, but also varied considerably depending on the plant-strain combination. The variation was partly explained by fungal traits shared by different lineages, like growth rates or melanization. The origin of strains also affected their symbioses, with endophytes isolated from Microthlaspi spp. populations being more detrimental to M. erraticum than strains from other sources. Our findings suggest that plant-endophyte associations are subject to local processes of selection, in which particular combinations of symbionts are favored across landscapes. We also show that different common endophytic taxa have differential sets of traits found to affect interactions, hinting to a functional complementarity that can explain their frequent co-existence in natural communities.

    https://doi.org/10.1038/ismej.2016.140
  • Mycological Progress
    01-12-2016

    Low diversity and abundance of root endophytes prevail throughout the life cycle of an annual halophyte

    Jose Maciá Vicente, Thomas Nau, Meike Piepenbring

    Plants growing in highly saline soils harbor unique communities of fungal root endophytes. We aimed to gain insight into how these communities are established in natural plant populations. We used cultivation-based and molecular approaches to examine root-endophytic colonization in the annual halophyte Salicornia patula at three time points over a 5-month period, from establishment to flowering. At the last sampling, the endophytic community of S. patula was compared to that in the related but perennial halophyte Arthrocnemum macrostachyum. The presence of root endophytes in S. patula was negligible at the first two sampling times, and remained low at the last sampling compared to A. macrostachyum. The latter species showed a well-established endophytic community in its roots that differed from that in S. patula, which was dominated by members of Pleosporales. Although such differences could be partially due to the host lifestyle, the possibility of a strong effect of the substratum could not be excluded. Altogether, our data indicate that the fungal endophytic colonization of roots is a slow process under salt stress. Therefore, we suggest that, in contrast to what is proposed for other systems, endophyte symbioses are unlikely to impact the development of the short-life-cycled S. patula living in these environments.

    https://doi.org/10.1007/s11557-016-1241-5
  • Environmental Microbiology
    01-09-2016

    The local environment determines the assembly of root endophytic fungi at a continental scale

    Kyriaki Glynou, Tahir Ali, Ann Katrin Buch, Sevda Haghi Kia, Sebastian Ploch, Xiaojuan Xia, Ali Çelik, Marco Thines, Jose Maciá Vicente

    Root endophytic fungi are found in a great variety of plants and ecosystems, but the ecological drivers of their biogeographic distribution are poorly understood. Here, we investigate the occurrence of root endophytes in the non-mycorrhizal plant genus Microthlaspi, and the effect of environmental factors and geographic distance in structuring their communities at a continental scale. We sampled 52 plant populations across the northern Mediterranean and central Europe and used a cultivation approach to study their endophytic communities. Cultivation of roots yielded 2601 isolates, which were grouped into 296 operational taxonomic units (OTUs) by internal transcribed spacer sequencing of 1998 representative colonies. Climatic and spatial factors were the best descriptors of the structure of endophytic communities, outweighing soil characteristics, host genotype and geographical distance. OTU richness was negatively affected by precipitation, and the composition of communities followed latitudinal gradients of precipitation and temperature. Only six widespread OTUs belonging to the orders Pleosporales, Hypocreales and Helotiales represented about 50% of all isolates. Assessments of their individual distribution revealed particular ecological preferences or a cosmopolitan occurrence. Our findings support a strong influence of the local environment in determining root endophytic communities, and show a different niche occupancy by individual endophytes.

    https://doi.org/10.1111/1462-2920.13112
  • Phytochemistry
    01-07-2016

    Distinguishing commercially grown Ganoderma lucidum from Ganoderma lingzhi from Europe and East Asia on the basis of morphology, molecular phylogeny, and triterpenic acid profiles

    Florian Hennicke, Zakaria Cheikh‐Ali, Tim Liebisch, Jose Maciá Vicente, Helge B Bode, Meike Piepenbring

    In China and other countries of East Asia, so-called Ling-zhi or Reishi mushrooms are used in traditional medicine since several centuries. Although the common practice to apply the originally European name 'Ganoderma lucidum' to these fungi has been questioned by several taxonomists, this is still generally done in recent publications and with commercially cultivated strains. In the present study, two commercially sold strains of 'G. lucidum', M9720 and M9724 from the company Mycelia bvba (Belgium), are compared for their fruiting body (basidiocarp) morphology combined with molecular phylogenetic analyses, and for their secondary metabolite profile employing an ultra-performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESIMS) in combination with a high resolution electrospray ionization mass spectrometry (HR-ESI-MS). According to basidiocarp morphology, the strain M9720 was identified as G. lucidum s.str. whereas M9724 was determined as Ganoderma lingzhi. In molecular phylogenetic analyses, the M9720 ITS and beta-tubulin sequences grouped with sequences of G. lucidum s.str. from Europe whereas those from M9724 clustered with sequences of G. lingzhi from East Asia. We show that an ethanol extract of ground basidiocarps from G. lucidum (M9720) contains much less triterpenic acids than found in the extract of G. lingzhi (M9724). The high amount of triterpenic acids accounts for the bitter taste of the basidiocarps of G. lingzhi (M9724) and of its ethanol extract. Apparently, triterpenic acids of G. lucidum s.str. are analyzed here for the first time. These results demonstrate the importance of taxonomy for commercial use of fungi.

    https://doi.org/10.1016/j.phytochem.2016.03.012
  • Mycological Progress
    01-02-2016

    A new species of Exophiala associated with roots

    Jose Maciá Vicente, Kyriaki Glynou, Meike Piepenbring

    A new species of the genus Exophiala (Herpotrichiellaceae, Ascomycota), Exophiala radicis, is described. The description is based on five strains isolated as endophytes from roots of the brassicaceous plant Microthlaspi perfoliatum s.l., collected at different localities in Europe. As evidenced by phylogenetic analyses of regions of the ribosomal DNA [the small and large subunits, and the internal transcribed spacers (ITS)] and the translation elongation factor 1-α, the β-tubulin, and the actin genes, the new species is closely related to Exophiala tremulae and Exophiala equina. E. radicis differs from E. tremulae morphologically by the shape and size of their conidia. A comparison of ITS sequences of E. radicis with GenBank records suggests that the species has a wide distribution in the northern hemisphere, and that it is commonly associated with living plant roots, indicating potential adaptations to this substrate.

    https://doi.org/10.1007/s11557-016-1161-4
  • Phytochemistry
    01-10-2015

    Diversity of exophillic acid derivatives in strains of an endophytic Exophiala sp.

    Zakaria Cheikh‐Ali, Kyriaki Glynou, Tahir Ali, Sebastian Ploch, Marcel Kaiser, Marco Thines, Helge B Bode, Jose Maciá Vicente

    Members of the fungal genus Exophiala are common saprobes in soil and water environments, opportunistic pathogens of animals, or endophytes in plant roots. Their ecological versatility could imply a capacity to produce diverse secondary metabolites, but only a few studies have aimed at characterizing their chemical profiles. Here, we assessed the secondary metabolites produced by five Exophiala sp. strains of a particular phylotype, isolated from roots of Microthlaspi perfoliatum growing in different European localities. Exophillic acid and two previously undescribed compounds were isolated from these strains, and their structures were elucidated by spectroscopic methods using MS, 1D and 2D NMR. Bioassays revealed a weak activity of these compounds against disease-causing protozoa and mammalian cells. In addition, 18 related structures were identified by UPLC/MS based on comparisons with the isolated structures. Three Exophiala strains produced derivatives containing a β-d-glucopyranoside moiety, and their colony morphology was distinct from the other two strains, which produced derivatives lacking β-d-glucopyranoside. Whether the chemical/morphological strain types represent variants of the same genotype or independent genetic populations within Exophiala remains to be evaluated.

    https://doi.org/10.1016/j.phytochem.2015.08.006
  • Microbial Ecology
    10-2012

    Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

    Jose Maciá Vicente, Valeria Ferraro, Santella Burruano, Luis V. Lopez-Llorca

    Structure of fungal communities is known to be influenced by host plants and environmental conditions. However, in most cases, the dynamics of these variation patterns are poorly understood. In this work, we compared richness, diversity, and composition between assemblages of endophytic and rhizospheric fungi associated to roots of two plants with different lifestyles: the halophyte Inula crithmoides and the non-halophyte I. viscosa (syn. Dittrichia viscosa L.), along a spatially short salinity gradient. Roots and rhizospheric soil from these plants were collected at three points between a salt marsh and a sand dune, and fungi were isolated and characterized by ITS rDNA sequencing. Isolates were classified in a total of 90 operational taxonomic units (OTUs), belonging to 17 fungal orders within Ascomycota and Basidiomycota. Species composition of endophytic and soil communities significantly differed across samples. Endophyte communities of I. crithmoides and I. viscosa were only similar in the intermediate zone between the salt marsh and the dune, and while the latter displayed a single, generalist association of endophytes, I. crithmoides harbored different assemblages along the gradient, adapted to the specific soil conditions. In the lower salt marsh, root assemblages were strongly dominated by a single dark septate sterile fungus, also prevalent in other neighboring salt marshes. Interestingly, although its occurrence was positively correlated to soil salinity, in vitro assays revealed a strong inhibition of its growth by salts. Our results suggest that host lifestyle and soil characteristics have a strong effect on endophytic fungi and that environmental stress may entail tight plant-fungus relationships for adaptation to unfavorable conditions.

    https://doi.org/10.1007/s00248-012-0066-2
  • Annals of Applied Biology
    12-2009

    Colonisation of barley roots by endophytic Fusarium equiseti and Pochonia chlamydosporia

    Jose Maciá Vicente, L. C. Rosso, A. Ciancio, Hans Börje Jansson, Luis V. Lopez-Llorca

    Colonisation of plant roots by endophytic fungi may confer benefits to the host such as protection against abiotic or biotic stresses or plant growth promotion. The exploitation of these properties is of great relevance at an applied level, either to increase yields of agricultural crops or in reforestation activities. Fusarium equiseti is a naturally occurring endophyte in vegetation under stress in Mediterranean ecosystems. Pochonia chlamydosporia is a nematode egg-parasitic fungus with a worldwide distribution. Both fungi have the capacity to colonise roots of non-host plants endophytically and to protect them against phytopathogenic fungi under laboratory conditions. The aim of this study was to evaluate the root population dynamics of these fungi under non-axenic practical conditions. Both fungal species were inoculated into barley roots. Their presence in roots and effects on plant growth and incidence of disease caused by the pathogen Gaeumannomyces graminis var. tritici were monitored periodically. Both fungi colonised barley roots endophytically over the duration of the experiment and competed with other existing fungal root colonisers. Furthermore, colonisation of roots by P. chlamydosporia promoted plant growth. Although a clear suppressive effect on disease could not be detected, F. equiseti isolates reduced the mean root lesion length caused by the pathogen. Results of this work suggest that both F. equiseti and P. chlamydosporia are long-term root endophytes that confer beneficial effects to the host plant.

    https://doi.org/10.1111/j.1744-7348.2009.00352.x
  • Plant Signaling and Behavior
    05-2009

    Assessing fungal root colonization for plant improvement

    Jose Maciá Vicente, Hans Börje Jansson, Luis V. Lopez-Llorca
    https://doi.org/10.4161/psb.4.5.8393
  • New Phytologist
    05-2009

    Erratum

    Jose Maciá Vicente, Hans Börje Jansson, Nicholas J. Talbot, Luis V. Lopez-Llorca
    https://doi.org/10.1111/j.1469-8137.2009.02842.x
  • New Phytologist
    04-2009

    Real-time PCR quantification and live-cell imaging of endophytic colonization of barley (Hordeum vulgare) roots by Fusarium equiseti and Pochonia chlamydosporia

    Jose Maciá Vicente, Hans Börje Jansson, Nicholas J. Talbot, Luis V. Lopez-Llorca

    • New tools were developed for the study of the endophytic development of the fungal species Fusarium equiseti and Pochonia chlamydosporia in barley (Hordeum vulgare) roots. These were applied to monitor the host colonization patterns of these potential candidates for biocontrol of root pathogens. • Molecular beacons specific for either F. equiseti or P. chlamydosporia were designed and used in real-time polymerase chain reaction (PCR) quantification of fungal populations in roots. Genetic transformation of isolates with the green fluorescent protein (GFP) gene was carried out using an Agrobacterium tumefaciens-mediated transformation protocol, and spatial patterns of root colonization were investigated by laser confocal microscopy. • Quantification of endophytes by real-time PCR in roots of barley gave similar results for all fungi, and was more accurate than culturing methods. Conversely, monitoring of root colonization by GFP-expressing transformants showed differences in the endophytic behaviours of the two species, and provided evidence of a plant response against endophyte colonization. • Both F. equiseti and P. chlamydosporia colonized barley roots endophytically, escaping attempts by the host to prevent fungal growth within root tissues. This strongly supports a balanced antagonism between the virulence of the colonizing endophyte and the plant defence response. Development of real-time PCR techniques and GFP transformants of these fungal species will facilitate future work to determine their biocontrol capacity.

    https://doi.org/10.1111/j.1469-8137.2008.02743.x
  • Canadian Journal of Microbiology
    08-2008

    Colonization of barley roots by endophytic fungi and their reduction of take-all caused by Gaeumannomyces graminis var. tritici

    Jose Maciá Vicente, Hans Börje Jansson, Kurt Mendgen, Luis V. Lopez-Llorca

    Fungal root endophytes obtained from natural vegetation were tested for antifungal activity in dual culture tests against the root pathogen Gaeumannomyces graminis var. tritici. Fifteen isolates, including Acremonium blochii, Acremonium furcatum, Aspergillus fumigatus, Cylindrocarpon sp., Cylindrocarpon destructans, Dactylaria sp., Fusarium equiseti, Phoma herbarum, Phoma leveillei, and a sterile mycelium, selected based on the dual culture test, were inoculated on barley roots in growth tubes under axenic conditions, both in the absence and presence of G. graminis var. tritici. All isolates colonized the rhizosphere and very often the root cortex without causing disease symptoms and without affecting plant growth. Eight isolates significantly reduced the symptoms caused by G. graminis var. tritici, and 6 of them reduced its presence in the roots.

    https://doi.org/10.1139/W08-047
  • FEMS Microbiology Ecology
    04-2008

    Fungal root endophytes from natural vegetation in Mediterranean environments with special reference to Fusarium spp

    Jose Maciá Vicente, Hans Börje Jansson, Samir K. Abdullah, Enric Descals, Jesus Salinas, Luis V. Lopez-Llorca

    Surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the Alicante province (Southeast Spain). All plant species examined were colonized by endophytic fungi. A total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (ITS) and translation elongation factor-1α gene region (TEF-1α) sequencing] techniques. One hundred and forty-two fungal species were identified, belonging to 57 genera. Sterile mycelia were assigned to 177 morphospecies. Fusarium and Phoma species were the most frequent genera, followed by Aspergillus, Alternaria and Acremonium. Fungal root endophytic communities were influenced by the soil type where their respective host plants grew, but not by location (coastal or inland sites). Fusarium oxysporum, Aspergillus fumigatus and Alternaria chlamydospora contributed most to the differences found between endophytic communities from sandy and saline soils. Host preference was found for three Fusarium species studied. Fusarium oxysporum and Fusarium solani were especially isolated from plants of the family Leguminosae, while Fusarium equiseti showed a preference for Lygeum spartum (Gramineae). In some cases, specificity could be related to intra-specific variability as shown by sequencing of the TEF-1α in the genus Fusarium.

    https://doi.org/10.1111/j.1574-6941.2007.00443.x

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